Figure5
DerivatizedB100 samples resolvewell on the15m x0.32mmMXT®-Biodiesel TG column,which is factory
coupled toa0.53mm retentiongapusinganAlumaseal™ connector.
MetalColumnSolutions:TwoOptions for IncreasedStabilityandPerformance
• 0.32mmMXT®-Biodiesel TG columnwith a 0.53mm retention gap,
factory coupledwith anAlumaseal™ connector
• 0.53mmMXT®-Biodiesel TG columnwith a built-in0.53mm
Integra-Gap™ integrated retention gap
The primary advantage of usingmetalMXT® columns is that they aremore stable at high temperatures than fused silica columns.
Thismeans theywill exhibit lower bleed, improving analytical performance, andhave longer lifetimes,making them a cost-effective
option.Theyalso canbebrought tohigh temperatures (430°C) allowingnonvolatilematerial tobebakedoff of the column, remov-
ing carryover contamination and improving cycle times.
MetalMXT®-Biodiesel TG columns areoffered in the same columndimensions as their fused silica counterparts.Twodifferent col-
umn configurations are available for cool on-column injection: 1) a 10m (or 15m) x 0.32mm IDMXT®-Biodiesel TG column fac-
torycoupled toa2mx0.53mm retentiongapusinganAlumaseal™connector, and2) a14mx0.53mm IDMXT®-BiodieselTGcol-
umnwith a built-in2m x 0.53mm ID Integra-Gap™ integrated retention gap.
Target analytes resolvewell and the solvent and triglyceridepeaks show excellent symmetryonboth columns (Figures 5 and6), but
the0.53mmMXT®-BiodieselTGcolumnwith the Integra-Gap™ integrated retentiongapeliminates theneed foraconnector,mak-
ing connector-related leaks a thingof the past. Peak shape for butanetriol is very good, demonstrating inertness, and the resolution
and response for themono-,di-and triglycerides is excellent.The leak-proof 0.53mmMXT®-BiodieselTGcolumnwith the Integra-
Gap™ integrated retention gap is theultimate biodiesel solution (Figure 7).
0
10
20
Time (min)
Column:
MXT
®
-BiodieselTG, 15m, 0.32mm ID, 0.10µm (cat.# 70291) with
a 2m x 0.53mmMXT
®
retention gap connectedwith an
Alumaseal™ connector (17m total length)
Sample:
biodiesel (B100), derivatized
Inj.:
cool on-column injection
1µL in heptane
Inj. temp.:
oven track
Carrier gas:
hydrogen, constant flow
Flow rate:
3mL/min.
Oven temp.:
50°C (hold 1min.) to 180°C@ 15°C/min. to 230°C@ 7°C/min. to 380°C@
30°C/min. (hold 5min.)
Det.:
FID@ 380°C
GC_PC00968
Glycerin
butanetrial (IS)
tricaprine (IS)
Monoglycerides
Monoglycerides
Diglycerides
Triglycerides
