• 2 •
Pinnacle
™
DBC18 5µmColumns
1.0mm ID
2.1mm ID
3.2mm ID
4.6mm ID
Length
cat.#
cat.#
cat.#
cat.#
price
30mm
9414531
9414532
9414533
9414535
50mm
9414551
9414552
9414553
9414555
100mm
9414511
9414512
9414513
9414515
150mm
9414561
9414562
9414563
9414565
200mm
9414521
9414522
9414523
9414525
250mm
9414571
9414572
9414573
9414575
Pinnacle
™
DBC8 5µmColumns
1.0mm ID
2.1mm ID
3.2mm ID
4.6mm ID
Length
cat.#
cat.#
cat.#
cat.#
price
30mm
9413531
9413532
9413533
9413535
50mm
9413551
9413552
9413553
9413555
100mm
9413511
9413512
9413513
9413515
150mm
9413561
9413562
9413563
9413565
200mm
9413521
9413522
9413523
9413525
250mm
9413571
9413572
9413573
9413575
Pinnacle
™
DBCyano 5µmColumns
1.0mm ID
2.1mm ID
3.2mm ID
4.6mm ID
Length
cat.#
cat.#
cat.#
cat.#
price
30mm
9416531
9416532
9416533
9416535
50mm
9416551
9416552
9416553
9416555
100mm
9416511
9416512
9416513
9416515
150mm
9416561
9416562
9416563
9416565
200mm
9416521
9416522
9416523
9416525
250mm
9416571
9416572
9416573
9416575
Pinnacle
™
DB Silica 5µmColumns
1.0mm ID
2.1mm ID
3.2mm ID
4.6mm ID
Length
cat.#
cat.#
cat.#
cat.#
price
30mm
9410531
9410532
9410533
9410535
50mm
9410551
9410552
9410553
9410555
100mm
9410511
9410512
9410513
9410515
150mm
9410561
9410562
9410563
9410565
200mm
9410521
9410522
9410523
9410525
250mm
9410571
9410572
9410573
9410575
includes a C8 and a cyano bonded phase, and bare
silica. Each of the bonded phases is endcapped.
To ensure the same high quality and reliability as
offered by all other RestekHPLC columns, we
established demanding quality control procedures
to ensure each column performs as expected.
Figure 3 is an example analysis of the test mix we
use to individually quality check every Pinnacle
™
DB column.
The Pinnacle
™
DB line is available in a wide range
of column dimensions, from 1mm narrow bore
columns through 50mm preparative-scale columns.
Please call our Technical Service Team for addition-
al information about this new column line, or call
our Customer Service representatives to place an
order.
Figure 2
Excellent peak shapes for basic pharmaceuticals help ensure accurate quantification.
2
3
4
5
6
7
min.
1
2
3
4
Peak List:
Conc.
1. theobromine
225µg/mL
2. theophylline
418µg/mL
3. ß-hydroxyethyltheophylline 418µg/mL
4. caffeine
400µg/mL
Table I
Physical characteristics of Pinnacle
™
DB silica.
LC_0241
Particle:
5µm, spherical
Pore size:
140Å
Pore volume:
0.65mL/g
Carbon load:
C18 - 11%
C8 - 6%
cyano - 4%
Figure 3
Every Pinnacle
™
DBcolumnmust pass
stringent efficiency criteria.
To order
preparative-scale
Pinnacle
™
DB
columns, please contact our Technical Service
Team at 800-356-1688 or 814-353-1300, ext. 4
or contact your local Restek Representative.
LC_0249
Sample:
Reversed Phase TestMix
Inj.:
5µL
Sample Diluent:
methanol:water (75:25 v/v)
Column:
Pinnacle
™
DBC18
Catalog #:
9414565
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
140Å
Conditions:
Mobile Phase:
A: water
B: methanol
Isocratic: 20%A:80%B
Run Time:
8min.
Flow:
1.0mL/min
Temp.:
ambient
Det.:
UV@ 254nm
Peak List:
Conc.
1. uracil
0.02mg/mL
2. benzene
3.0mg/mL
3. naphthalene
0.5mg/mL
4. biphenyl
0.06mg/mL
Sample:
Inj.:
2µL
Sample Diluent: mobile phase
Column:
Pinnacle
™
DBC18
Catalog #:
9414565
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
140Å
Conditions:
Mobile Phase: A:20mMolar
KH
2
PO
4
buffer, pH 3.0
B:acetonitrile
Isocratic: 90%A:10%B
Flow:
1.0mL/min
Temp.:
27°C
Det.:
UV@ 254nm
