with data pack
with data pack
Figure 2
AnUltra Carbamate column provides rapid, excellent results in LC/MS analyses.
Figure 1
AnUltra Carbamate column reduces
analysis time by 70%.
• 10 •
800-356-1688
The traditional HPLC column for analyses of carba-
mates is a 250 x 4.6mm column containing a 5µm
C18 packing. This column separates 10 target carba-
mates and an internal standard, but the price paid
for the separation is steep—the analysis time is
approximately 35minutes. Several columnmanufac-
turers havemanaged to reduce the analysis time by
packing a 4µm C18 particle in a 150mm column.
This configuration reduces the analysis time to
approximately 25minutes.
byGregFrance, HPLCMarketingManager, RebeccaWittrig, Ph.D., Food, Flavor,&Fragrance InnovationsChemist,
andVernonBartlett, HPLCInnovationsManager
NewUltraHPLCColumn for
CarbamatesAnalysis
Unique Stationary Phase Separates
10Carbamates in 10Minutes
✔
Reduce analysis time by 70% versus a C18 column.
✔
Greatly reduce solvent purchase and disposal costs.
✔
Compatiblewith LC/MS detection.*
Changing the physical parameters of a column is a
brute force way to improve analyte separation or
reduce analysis time. A better way to improve a
method is to examine the analytes and determine if
an alternative stationary phase ormobile phase
compositionwould provide a better or faster sepa-
ration. After the optimum stationary phase and
mobile phase are selected, the analysis can be fine-
tuned by adjusting the column length and/or the
particle size.
With a chemistry-based approach inmind, Restek
chemists developed the Ultra Carbamate column
specifically for carbamates analysis. The new col-
umn has a unique stationary phase that, when
bonded to 3µm silica, creates a packing that sepa-
rates 10 target carbamates in under 10minutes
(Figure 1). The new stationary phase is compatible
with LC/MS detection (Figure 2). Themobile phas-
es used to obtain these separations are binary gra-
dients consisting of water, methanol, and acetoni-
trile, without (Figure 1) or with ammonium for-
mate (Figure 2).
An Ultra Carbamate column can process asmany as
3 to 4 samples per hour, versus less than 2 samples
per hour on a traditional C18 column. In addition
to increased sample throughput, faster analyses sig-
nificantly reduce solvent usage—and the costs of
disposing of the solvent waste after the analysis. If
you have been analyzing carbamates with a general-
purpose C18 column, we think youwill be highly
impressedwith the performance of an Ultra
Carbamate column—and the increased sample
throughput and solvent savings will quickly pay for
your new column.
*Also compatiblewith post-column derivitization and fluores-
cence detection, if total system dead volume is less than 650µL,
inclusive of post-column reactors.
Column:
Ultra Carbamate
Catalog #:
9177355
Dimensions:
50 x 4.6mm
Particle size:
3µm
Pore size:
100Å
Conditions:
Inj.:
5µL
Conc.:
50µg/mL
Solvent:
methanol
Restek standards:
Catalog# 32274 (IS) and 32273mixed 50:50
Mobile Phase:
A: 90:10water:methanol
B: 90:10methanol:acetonitrile
Time (min):
%B
0
10
10
90
Flow:
1.5mL/min
Temp.:
27°C
Det.:
UV@220nm
LC_0225
1. aldicarb sulfone
2. aldicarb sulfoxide
3. oxamyl
4. methomyl
5. 3-hydroxycarbofuran
6. aldicarb
7. propoxur
8. carbofuran
9. carbaryl
10. methiocarb
11. 4-bromo-3,5-dimethyl-
phenyl-N-methyl-
carbamate (BDMC) (IS)
70%
faster!
Column:
Ultra Carbamate
Catalog #:
9177315
Dimensions:
100 x 4.6mm
Particle size:
3µm
Pore size:
100Å
Inj.:
10µL
Sample:
531.1 Carbamate
Pesticide CalibrationMix
(cat.# 32273)
Conc.:
100µg/mL each
Solvent:
methanol
Mobile Phase:
A: 90:10water:methanol + 10mM
ammonium formate
B: 10:90 acetonitrile:methanol + 10mM
ammonium formate
Gradient:
90:10 A:B to 10:90 A:B
from 0-15min.
Flow:
1mL/min. (0.75mL/min. to
UV, 0.25mL/min. toMS)
Temp.:
ambient
MS Conditions:
Det.:
Micromass ZMD
Mode:
ESI+
Capillary V:
3.50
Extractor:
4.0
Ion Energy:
0.4
Multiplier:
650
Source Temp.:
100ºC
Desolv. Temp.:
250ºC
Gas Flow:
490 L/hr.
Compound
Ion
Cone V
1. aldicarb sulfone
223.3
25V
2. aldicarb sulfoxide
207.3
18V
3. oxamyl
237.2*
10V
4. methomyl
163.2
15V
5. 3-hydroxycarbofuran 238.3
15V
6. aldicarb
191.2
8V
7. propoxur
210.2
18V
8. carbofuran
222.3
22V
9. carbaryl
202.2
18V
10. methiocarb
226.3
19V
*Ammonium adduct;
others [M+H]+ ions.
Ordering Information
| 3µmUltraCarbamate
HPLCColumns
Description
Length 4.6mm ID
Ultra Carbamate
50mm 9177355
Ultra Carbamate
100mm 9177315
aldicarb
aldicarb sulfone
aldicarb sulfoxide
carbaryl
carbofuran
3-hydroxycarbofuran
methiocarb
methomyl
oxamyl
propoxur (baygon)
100µg/mL each inmethanol, 1mL/ampul
531.1CarbamatePesticideCalibration
Mixture
(10 components)
Each
5-pk.
10-pk.
32273
32273-510
—
32273-500
32273-520
32373
4-bromo-3,5-dimethylphenyl-N-methylcarbamate (BDMC)
100µg/mL inmethanol, 1mL/ampul
Internal Standard
Each
5-pk.
10-pk.
32274
32274-510
—
32274-500
32274-520
32374
