the
in this issue
Leak Detective
™
II
…pg. 5
ShinCarbon ST Micropacked Columns
for Permanent Gases
…pg. 6
Optimized Analysis for GC/MS
Screening for Phenols
…pg. 7
NewAnalytical ReferenceMaterials
…pg. 8–9
Silcosteel
®
-CR Tubing and Fittings
…pg. 10
Pinnacle
II
™
PAH HPLCColumns
…pg. 11
Rt-2560 GC Columns and Reference
Materials for FAME Analysis
…pg. 12
Drilled Uniliner
®
GC Inlet Liner
…pg. 13
Peak Performers
…pg. 14
Cool Tools
…pg. 15
Mini Air Sampling Canisters
…pg. 15
Behind the Scenes
…pg. 16
2003
vol.1
Advantage
Pinnacle
™
DB
HPLC Columns
…pg. 1–2
EZ No-Vent
™
GC/MS
Connector
…pg. 3
Optimized Analysis for
Screening for Semivolatile
Organic Compounds
…pg. 4–5
Innovators of HighResolution
Chromatography Products
by Greg France, HPLCProductsMarketingManager
NewPinnacle
™
DBHPLCColumns
Superior Performance for Basic Compounds
✔
Silicamanufactured inRestek facilities, for total control of product quality.
✔
Uniquemanufacturing process ensures sharp, symmetric peaks for basic analytes.
✔
Columns available in narrow bore through preparative-scale formats.
In 2001 our Pinnacle II
™
line of columns put Restek
among the select fewHPLC columnmanufacturers
whomanufacture their own silica. We believe that to
truly control HPLC variables, amanufacturermust
control the entire columnmanufacturing process,
beginning at the initial step—making awell-charac-
terized, consistently-performing silica.
Now, we are pleased to introduce a new line of
HPLC columns, Pinnacle
™
DB columns, prepared
from our newest silica support. Pinnacle
™
DB silica
is a highly base-deactivated silica,
suitable for a wide range of
challenging applications. It
is ideal for analyses of
basic compounds, or
mixtures of varied func-
tionality. Part of our
manufacturing process
for Pinnacle
™
DB silica is
a unique processing step
that creates a base-deactivat-
ed particle with excellent per-
formance characteristics. The base
deactivation and attractivemass transfer capabilities
of the particles enable Pinnacle
™
DB silica to
resolve and elute basic compounds (e.g., pharma-
ceuticals) without severe peak tailing—withmini-
mal or no need formobile phasemodifiers such as
tetrabutylammonium (TBA). Physical characteris-
tics of Pinnacle
™
DB silica are listed in Table I
(page 2).
Columnmanufacturers often use an analysis of a
pyridine/phenol test mix to demonstrate a column's
separation capabilities and indicate the peak shape
that can be anticipated for basic compounds.
Figure 1 shows pyridine/phenol separations from a
Pinnacle
™
DB C18 and a non-base-deactivated C18
column. The sharp, symmetric peaks from the
Pinnacle
™
DB C18 column—without amobile
phasemodifier—are what an analyst can expect for
many pharmaceutical or other basic analytes.
Figure 2 shows an array of basic pharmaceutical
compounds analyzed, with excellent results, on a
Pinnacle
™
DB C18 column. Note the consistent
peak symmetry. Often, neutral or acidic compounds
can be present with basic pharmaceutical com-
pounds as impurities or degradation products.
Pinnacle
™
DB columns are highly suited to these
challenges as well. Along with themonomeric C18
bonded phase, the Pinnacle
™
DB line currently
Figure 1
Pinnacle
™
DBC18 columns provide sharp, symmetric peaks for basic analytes, without a
mobile phasemodifier.
LC_0247
Peak List:
Conc.
1. uracil
5.0µg/mL
2. pyridine
0.1µL/mL
3. phenol
1.86mg/mL
Sample:
Inj.:
5.0µL
Sample Diluent:
mobile phase
Conditions:
Mobile Phase:
A: 20mM potassium
phosphate, pH 7.0
B: acetonitrile
Isocratic: 80%A:20%B
Flow:
1.0mL/min
Temp.:
ambient
Det.:
UV@ 254nm
Column:
Pinnacle
™
DBC18
Catalog #:
9414565
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
140Å
Column:
Pinnacle
II
™
C18
Catalog #:
9214565
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
110Å
LC_0248
