• 11 •
Mutagenic, carcinogenic, and pervasive in themod-
ern environment, polynuclear aromatic hydrocar-
bons (PAHs) are emitted bymany sources of com-
bustion, including heating devices that burn fossil
fuels and internal combustion engines inmotor
vehicles. Because they are universal, PAHs are ana-
lyzed inmany air, water, and soil matrices.
Environmental samples often contain complexmix-
tures of PAHs that are difficult to resolve, due to
considerable structural similarity among the various
ReproducibleAnalyses of
Polynuclear AromaticHydrocarbons
UsingPinnacle
II
™
PAHHPLCColumns and
RestekReferenceStandards
by Terrence S. Reid, Innovations TeamChemist
✔
Special-purposeHPLC columns resolve 16PAHs to baseline.
✔
In-house-manufactured silica ensures reproducible column performance.
✔
Three reference standards—choose the concentrations best suited to your need.
Pinnacle
II
™
PAH 5µmColumns
2.1mm ID
3.2mm ID
4.6mm ID
Length
cat.#
cat.#
cat.#
price
100mm
—
9219513
9219515
150mm
—
9219563
9219565
200mm
—
9219523
9219525
250mm
9219572
9219573
9219575
acenaphthene
acenaphthylene
anthracene
benzo(a)anthracene
benzo(a)pyrene
benzo(b)fluoranthene
benzo(k)fluoranthene
benzo(ghi)perylene
chrysene
dibenzo(a,h)anthracene
fluoranthene
fluorene
indeno(1,2,3-cd)pyrene
naphthalene
phenanthrene
pyrene
2,000µg/mL each inmethylene chloride, 1mL/ampul
SVCalibrationMix #5
(16 components)
3/90 and 4/89 SOW
Each
5-pk.
10-pk.
31011
31011-510
—
w/data pack
31011-500
31011-520
31111
acenaphthene
1000µg/mL
acenaphthylene
1000
anthracene
1000
benzo(a)anthracene
500
benzo(a)pyrene
500
benzo(b)fluoranthene
500
benzo(k)fluoranthene
500
benzo(ghi)perylene
500
chrysene
500
dibenzo(a,h)anthracene 500
fluoranthene
500
fluorene
1000
indeno(1,2,3-cd)pyrene 500
naphthalene
1000
phenanthrene
500
pyrene
500
Inmethylene chloride, 1mL/ampul
610PAHCalibrationMix A
(16 components)
Each
5-pk.
10-pk.
31264
31264-510
—
w/data pack
31264-500
31264-520
31364
acenaphthene
1000µg/mL
acenaphthylene
2000
anthracene
100
benzo(a)anthracene
100
benzo(a)pyrene
100
benzo(b)fluoranthene
200
benzo(k)fluoranthene
100
phenanthrene
100
benzo(ghi)perylene
200
chrysene
100
dibenzo(a,h)anthracene 200
fluoranthene
200
fluorene
200
indeno(1,2,3-cd)pyrene 100
naphthalene
1000
pyrene
100
Inmethylene chloride:methanol (1:1), 1mL/ampul
610PAHCalibrationMix B
(16 components)
Each
5-pk.
10-pk.
31455
31455-510
—
w/data pack
31455-500
31455-520
31555
Figure 1
Pinnacle
II
™
PAH columns provide reproducible, baseline resolution of the 16 PAHs listed inUS
EPAMethod 610.
Sample:
Inj.:
610PAHCalibrationMix A (cat.# 31264), 5µL
Sample diluent: methylene chloride:acetonitrile (1:9, v/v)
Column:
Catalog #:
9219563
Dimensions:
150 x 3.2mm
Particle size:
5µm
Pore size:
110Å
Conditions:
Mobile phase: A: water; B: acetonitrile
Time:
%B
0
40
30
100
40
100
41
40
51
40
Flow:
0.5mL/min
Temp.:
30°C (or ambient)
Det.:
UV@ 254nm
LC_0243
Ret. Time (min.)
Conc.
Peak List:
Lot 1 Lot 2 Lot 3 (µg/mL)
1. naphthalene
11.95 12.17 11.80 100
2. acenaphthylene
13.41 13.65 13.26 100
3. acenaphthene
15.61 15.89 15.45 100
4. fluorene
16.09 16.31 15.92 100
5. phenanthrene
17.41 17.61 17.23 50
6. anthracene
18.80 18.92 18.61 100
7. fluoranthene
20.23 20.39 20.03 50
8. pyrene
21.31 21.46 21.11 50
9. benzo(a)anthracene
24.88 24.87 24.65 50
10. chrysene
25.64 25.54 25.40 50
11. benzo(b)fluoranthene 28.62 28.53 28.35 50
12. benzo(k)fluoranthene 29.97 29.74 29.70 50
13. benzo(a)pyrene
31.26 31.02 30.97 50
14. dibenzo(a,h)anthracene 33.09 32.74 32.81 50
15. benzo(ghi)perylene
34.46 34.24 34.16 50
16. indeno(1,2,3-cd)pyrene 35.19 34.85 34.91 50
analytes. Reversed phase HPLC is the favored
approach for analyses of PAHs because this tech-
nique can discriminate among the closely related
compounds, on the basis of molecular shape. US
Environmental Protection AgencyMethod 610, for
example, calls for reversed phase HPLC for analyses
of the 16 PAHs listed in Figure 1.
We developed the newest addition to our Pinnacle
II
™
HPLC product line, the Pinnacle II
™
PAH col-
umn, specifically for these demanding analyses. The
Pinnacle II
™
PAH stationary phase is a specialized
polymeric C18 bonding that uses unique shape
selectivity to provide baseline resolution of all 16
PAHs in EPAMethod 610. Further, wemake
Pinnacle II
™
PAH columns using silicawemanufac-
ture ourselves, at our Bellefonte, PA facility. Bymak-
ing our own silica, we have greater control over
both quality and reproducibility. Pinnacle II
™
PAH
columns provide the same selectivity as our original
Pinnacle
™
PAH columns, but greater reproducibility.
Lot-to-lot reproducibility of performance for
Pinnacle II
™
PAH columns is excellent, as shown in
Figure 1. Each of the three lots of columns repre-
sented has provided baseline resolution of the 16
PAHs listed inMethod 610, using a simple, linear
water/acetonitrilemobile phase gradient.
In addition to these special-purposeHPLC columns,
Restekmanufactures three analytical referencemixes
for EPAMethod 610. Themixes differ in concentra-
tions, but each contains all 16 listed PAHs, packaged
in convenient 1mL ampuls. The sample used to
obtain Figure 1 is 610 PAH CalibrationMix A.
If you are analyzing PAHs, the information present-
ed here shows Restek can provide both the reliable,
cost-effective HPLC columns and the chemical stan-
dards you need.
(Mix A)
lot 1
lot 2
lot 3
