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Figure 2: Increase efficiency and decrease analysis time, with lower pressure, by switching from FPP to Raptor™ SPP columns.
More on SPP and FPP
Read more on our work comparing SPP
and FPP or 2.7 and 5 µm SPP columns:
Look under “Resources” at
Selectivity Accelerated
SPP speed. USLC® resolution.
A new species of column.
The Raptor™ Suite of Innovative
Reversed-Phase Columns
Biphenyl • ARC-18 • C18
Order at
Si
O
Si CH3 CH 3
O
Si
TMS TMS
O
0.0
1 2
3
4
1
2
1
2
3
3
4
4
2.5 5.0 7.5 10.0
Time (min)
Time (min)
Time (min)
12.5 15.0 17.5
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5
0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5
Fully porous 5 µm
N = 12,940
Analysis time = 13.5 min
Pressure = 45 bar
Raptor™ Biphenyl SPP 5 µm
N = 18,516
Analysis time = 7.5 min
Pressure = 55 bar
Fully porous 3 µm
N = 17,835
Analysis time = 14.0 min
Pressure = 107 bar
LC_GN0556
Column
Temp.: 30 °C
Sample LC Reversed Phase Test Mix #1 (cat.# 35005)
Diluent: Methanol:water (75:25)
Inj. Vol.: 2 µL
Mobile Phase
A: Water
B: Acetonitrile
Time Flow
(min) (mL/min) %A %B
0.00 0.8 45 55
20 0.8 45 55
Detector DAD@ 254 nm
Instrument HPLC
Notes All columns were 150 mm x 4.6 mm ID. Values
for efficiency (N) were calculated from the
Biphenyl peak.
Peaks Conc.
(mg/mL)
1. Uracil 0.02
2. Benzene 3.0
3. Naphthalene 0.5
4. Biphenyl 0.06
Experience Selectivity Accelerated
Whether 2.7 or 5 µmdiameter particles are better for your application,
rugged Raptor™ SPP LC columns can give you the increased speed
and resolution you have been looking for. Experience Selectivity
Accelerated by visiting and ordering your
Raptor™ SPP LC columns today. You can also contact your local
Restek® representative ( to set up an
in-depth consultation.