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Figure 3 An amine modifier improves peak shape and changes
selecti vity fo r SSRls on an Allure" Basix column.
Peak List:
Ret Time
(min.) Asymmetry Selectivity«(X)
1.17
1.15 1.73
1.22 1.15
l. uracil
2. impurity
3. fluvoxamine maleate 3.754
4. lIuoxetine 5.553
5. sertralineHCI 6.222
U \.
" I i II, ii,II I"" I I '" ''1'' '' ' illi " " ,ii 'l " "\ ii I" """ 1" " 1111
1.0 2.0 3.0 4~ ~ M ~ U U M~
lCPH0354
sample:
Inj.: lOpL
Cone.: 100pg/mL each component
Sample diluent: water:acetonitnle, 50:50
Column:
Dimensions:
Particle size:
Pore size:
Conditions:
Mobile phase:
Flow:
Temp.:
Det.:
Allure~ Basix (cat.# 9161565)
150 x 4.6 mm
5!J~
60A
1%triethylamine in water (pH 6):acetonitrile, 50:50
I mUmin.
30°C
UV@23 0 nm
shape in reversed phase mod e. However, amine
additives work by blocking ioni zable silanols,
which can vary from column to column, so be sure
the amine concentration is high enough to sup­
press all potential silanol effects.
The Ultra PFP phase shows the best performance
at an acidic pH (Figure 4).
These analyses reveal that polar stationary phases
can effectively replace IPC in ana lyses of SSRls.
Overall, in RPC, polar stationary phases provide
better peak shape than alkyl ph ases for basic ana­
Iytes. When analyzing SSRls at neutral pH , the
Allure?" Basix phase is a good choice. When ana­
lyzing SSRls at an acidic pH , the Ultra PFP phase
is the better candidate.
Allure™ Basix Column
Column, 4.6mm
150mm
cal #
9161565
Ultra PFP Column
Column, 4.6mm
150mm
cat. #
9176565
ordering note
To order a 2.1mm, 3.2mm, or 4.6mm ID column with a Trident"
I ntegral Inlet Fitting, add "-700"to the catalog number for the
column.
Nominal additional charge
For guard cartridges and XG-XF guard cartridge fittings for these Figure 4 An Ultra PFP column shows good performance at acid ic pH.
columns, visit our website at
Ret. Time
Peak List: (min.) Asymmetry selectivity «(X)
l. uracil
2. impurity
3. fluvoxamine maleate 6.291 l.02
4. sertraline HCI 8.030 1.10 1.39
5. fluoxetine 9.142 l.01 1.17
sample:
Inj.: 10!JL
Cone.: 100!Jg/ mL each component
Sample diluent: water:acetonitrile, 50:50
Column: Ultra PFP (cat.# 9176565)
Dimensions: 150 x 4.6mm
Particle size: 5!Jm
Pore size: 100A
Conditions:
Mobilephase: 20mM potassiumphosphate monobasic in water (pH3):acetonitrile,70:30
Flow: I mL/min.
Temp.: 30°C
Det.: UV @ 230 nm
2006 vol. 1 • 11 •
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