ADV06-15_1-412

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Figure 1:

Raptor™ LC columns give you fast analysis times for cannabinoids without the expense of UHPLC equipment.

Figure 2:

Determine critical cannabinoids in minutes by GC using an Rxi®-35Sil MS column.

tech

tip

To see how the Rxi®-35Sil MS

outperforms traditional 5-type columns,

access our full technical article at

www.restek.com/ADV1516

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for Medical Cannabis Labs

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• Your continued success is our goal—we

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Whether you are using LC or GC for cannabis potency analysis, Restek

can provide the products and expertise you need to obtain accurate

results quickly. Use the methods shown here for analyzing the full

spectrum of acid and neutral cannabinoids using LC with minimal

capital investment, or get extremely fast, reliable, cost-effective

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1.75

min

100

105

110

Time (sec)

GC_FS0527

Column

Rxi®-35Sil MS, 15 m, 0.25 mm ID, 0.25 µm (cat.# 13820)

Sample

Cannabis sample 94

Injection

Inj. Vol.:

1 µL split (split ratio 10:1)

Liner:

Sky® 4 mm Precision® liner w/wool (cat.# 23305.5)

Inj. Temp.:

250 °C

Split Vent Flow Rate: 25 mL/min

Oven

Oven Temp.:

225 °C (hold 0.1 min) to 330 °C at 35 °C/min (hold 0.9 min)

Carrier Gas

, constant flow

Flow Rate:

2.5 mL/min

Detector

FID@ 350 °C

Constant Column

+ Constant Make-up: 50 mL/min

Make-up Gas Type:N

Hydrogen flow:

40 mL/min

Air flow:

450 mL/min

Data Rate:

20 Hz

Instrument

Agilent/HP6890 GC

Peaks

(sec)

Conc. (%)

1. Cannabichromene

82.0

2. Cannabidiol

84.6

5.7

3. delta-8-Tetrahydrocannabinol

93.5

4. delta-9-Tetrahydrocannabinol

97.1

2.0

5. Cannabigerol

99.4

6. Cannabinol

104.9

Time (min)

8 9

0.20

0.00 0.40 0.60 0.80 1.00 1.20 1.40 1.60 1.80 2.00 2.20 2.40 2.60 2.80 3.00 3.20 3.40 3.60 3.80 4.00

Peaks

1. Cannabivarin (CBDV)

2. Cannabidiolic acid (CBDA)

3. Cannabigerol (CBG)

4. Cannabidiol (CBD)

5. Tetrahydrocannabivarin (THCV)

6. Cannabinol (CBN)

7. delta-9-Tetrahydrocannabinol (∆9-THC)

8. Cannabichromene (CBC)

9. delta-9-Tetrahydrocannabinolic acid A (THCA)

Column

Raptor™ ARC-18 (cat.# 9314A65)

Dimensions:

150 mm x 4.6 mm ID

Particle Size:

2.7 µm

Temp.:

50 °C

Sample

Cannabidiolic acid (cat.# 34094)

Cannabigerol (cat.# 34091)

Cannabidiol (cat.# 34011)

Cannabinol (cat.# 34010)

delta-9-Tetrahydrocannabinol (THC) (cat.# 34067)

Cannabichromene (cat.# 34092)

delta-9-Tetrahydrocannabinolic acid A (THCA)

(cat.# 34093)

Diluent:

50:50 Methanol:water

Conc.:

50 µg/mL

Inj. Vol.:

5 µL

Mobile Phase

0.1% Formic acid in water

0.1% Formic acid in acetonitrile

Time (min) Flow (mL/min) %A %B

0.00

1.5

25 75

4.00

1.5

0 100

4.01

1.5

25 75

7.00

1.5

25 75

Detector

UV/Vis @ 220 nm

Instrument

HPLC

LC_GN0553