ADV06-15_1-412

141 / 413

Figure 2 Only the Biphenyl phase retains both test probes to
k' > 2, the level recommended to ensure separation from unretained
matrix contaminants.
2. tenoxicam
More retention with < V2 the
carbon load, compared to
phenyl hexyl columns
~. Pinnacle' DBBiphenyl
K'=l 1 K'=2
2
3
A minimum k' value of 2
is generally recommended
to fully separate target
analytes from matrix
contaminants.
0 1 2 3 4 5 6 7 8
L­
9 10min. lC_PH0478A
B. phenyl
1 PeakList:
l. uracil (void marker)
2. tenoxicam
3. sulinpyrazone
2
Sample:
Iru.:
3 Conc.:
Sample diluent:
0 1 2 3 4 5 6 7 8 9
C. phenyl hexyl
1
Column:
Dimensions:
Particlesize:
Poresize:
3
~
0 1 2 3 4 5 6 7 8 9
0.C18 Conditions:
Mobilephase:
1
2 3
Temp.:
Det.:
10IJL
l OOlJg/mL each component
40:60 formic acid:methanol
10min. lC_PH0478B
A: Pinnacle™DB Biphenyl (cat.# 9409565)
B: phenyl
C: phenyl hexyl
D: C18
l S0mm x 4.6mm
SlJm
14011
10min. lCPH0478C
A: waterwi 0.1%formic acid
B: methanol
Time(min) Flow (mt/min) %B
0.0 l. 0 60
2.0 l. 0 60
8.0 l. 0 90
20.0 l. 0 90
20.1 l. 0 60
30'C
Shimadzu PDA (SPD-M20A) @ 2S4nm
Table I Biphenyl columns show improved
retention of sulfone- and sulfoxide­
containing aromatic drugs.
K'Value
Biphenyl Phenyl hexyl Phenyl CI8
Tenoxicam 2.23 1.32 0.63.Z_ _0.23.~_
Sulfinpyrazone 4.18 3:9_0 l.88 l.89
Product Listing
Pinnacle' DB Biphenyl Columns (USP L11)
particle size: 1.9Ilm, 311m
or Sum, spherical
por e size: l40A
carbon load: 8%
;JJ,!m Column,.-'1"".0"'-m"'-m'-30mm
endcap : yes
pH range: 2.5 to 7.5
temperature limit: 80aC
cat. #
.,=~_,__----
9409331
50mm 9409351
100mm 9409311
150mm 9409361
cat. # 3i,Im Column,-'2.=1"'-m"'-m'-- -=~_,__----
30mm 9409332
50mm 9409352
100mm 9409312
150mm 9409362
;JJ,!mColumn 3.2mm cat.#
30mm 9409333
50mm 9409353
100mm 9409313
150mm 9409363
;3j.(rn Column, ~4"' cat. # _ .6""m""m'____ ~"-.!!...
30mm 9409335
50mm 9409355
100mm 9409315
150mm 9409365
~m Column lOmm cat. #
30mm 9409531
50mm 9409551
9409511 100m'!'.m'C- ~~"_----
150mm 9409561
200mm 9409521
250mm 9409571
~m Column, 2.1mm cat. #
30mm 9409532
50mm 9409552
100mm 9409512
150mm 9409562
200mm 9409522
250mm 9409572
cat. #
9409533
50mm 9409553
100mm 9409513
150mm 9409563
200mm 9409523
250mm 9409573
5f!mColumn, 4.6m".'m cat. #
~~=__----
30mm 9409535
~Omm 9409555
100mm 9409515
150mm 9409565
200mm 9409525
250mm 9409575
o 2 3 4 5 6 7 8 9 10min. lC PH0478D
Global RESTEK Advantage • 11 •
Website : E-mail : TelNo : 03 9762 2034 . . . in AUSTRALIA