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• 2 • 800-356-1688 • 2005 vol. 1
In addition to our Viva™ 300Å silica, we evaluated
300Å materials from five other vendors, and one
200Å material, determining pore characteristics
and surface area for each. We used nitrogen
gas porositometry, BET measurements1 , and
BJH calculation 2 to determine the surface area,
resented by 200-300Å pores. None of the other
materials evaluated comes close to the 67.3%
value obtained for Viva™ 300Å silica.
In selecting a wide pore material, it is important
to know the available surface area, the pore
volume, and the pore diameter distribution,
because these are the critical factors in deter-
mining retention. The exceptionally large avail-
able surface area of Viva ™ 300Å silica, and a
highly desirable pore volume and pore diameter
distribution, will help ensure effective retention
of peptides, proteins, or other large molecules,
making Viva™ 300Å products an excellent choice
for your analyses.
References
1. Barton, T., et al., Tailored Porous MaterialsChem. Mater.
11: 2633-2656 (1999).
2. Webb, P.A. and C. Orr, Analytical Methods in Fine Particle
TechnologyMicrometrics, Georgia, 1997, pp. 53-152.
pore volume, and pore diameter distribution of
each material. Figures 2 and 3 show the pore
volume and pore area for each material; Table I
indicates the available surface area for a given
pore diameter range. Viva™ 300Å silica shows,
by far, the greatest available surface area rep-
Figure 2 Pore volume vs pore diameter for commercial wide pore silicas (BJH desorption).
Only Viva™ silica has a sharp distribution around 300Å. (Change in scale for plots at right.)
Figure 3 Pore area vs pore diameter for commercial wide pore silicas (BJH desorption).
Viva™ silica shows a highly desirable distribution. (Change in scale for plots at right.)
Viva™Wide Pore C18 Columns 3.2mm ID 4.6mm ID
Length cat.# price cat.# price
5µm Columns
100mm 9514513 $380 9514515 $390
150mm 9514563 $400 9514565 $410
250mm 9514573 $465 9514575 $475
Viva™ Wide Pore Silica Columns
ordering note
For guard columns for Viva™ wide pore columns, refer
to our current catalog, or visit our website.
To order a 3.2mm or 4.6mm ID column with a Trident ™
Integral Inlet Fitting, add “-700” to the catalog number
for the column.
Nominal additional charge $15.00
Example:
100mm x 4.6mm ID Viva™ Wide Pore C18 column with
Trident™ Integral Inlet Fitting: 9514515-700
3.2mm ID 4.6mm ID
Length cat.# price cat.# price
5µm Columns
100mm 9510513 $380 9510515 $390
150mm 9510563 $400 9510565 $410
250mm 9510573 $465 9510575 $475
free literature
HPLC Columns and
Accessories
General-purpose and unique special-purpose
columns, guard columns, column kits, and bulk
materials, plus instrument parts, innovative
tools, accessories, and many example chro-
matograms. Our five silicas have characteristics
tailored to meet specific analytical requirements.
Request lit. cat.# 59241B (100 pages).
1 - “B” 200Å
2 - “B” 300Å
3 - 300Å
4 - Restek 300Å
4 - Restek 300Å
5 - 300Å
6 - 300Å
7 - 300Å
4 - Restek 300Å
5 - 300Å
6 - 300Å
7 - 300Å
1 - “B” 200Å
2 - “B” 300Å
3 - 300Å
4 - 300Å
Viva™ wide pore silica packings are available in bulk. Please inquire: 800-356-1688 or 814-353-1300,
ext. 4, or contact your Restek representative.
Pore Diameter (Å) Pore Diameter (Å)
Pore Diameter (Å) Pore Diameter (Å)