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• 5 •
Table I
Organochlorine pesticides and
retention times in GCxGC separation.
T
R
(sec.)
Analyte
Dimension 1 Dimension 2
1. tetrachloro-
m
-xylene
294
1.9
2.
α
-BHC
342
2.44
3. ß-BHC
378
2.96
4.
γ
-BHC
384
2.66
5.
δ
-BHC
420
2.94
6. heptachlor
480
2.52
7. aldrin
534
2.58
8. heptachlor epoxide
606
3.16
9.
γ
-chlordane
648
2.96
10. endosulfan I
672
3.32
11.
α
-chlordane
678
2.96
12. dieldrin
720
3.34
13. 4,4'-DDE
732
2.72
14. endrin
756
3.46
15. endosulfan II
780
3.78
16. 4,4'-DDD
810
3.18
17. endrin aldehyde
816
4.5
18. endosulfan sulfate
864
5.2
19. 4,4'-DDT
882
2.96
20. endrin ketone
942
4.68
21. methoxychlor
1008
2.82
22. decachlorobiphenyl
1320
2.62
Table II
Pesticide recovery values for a spiked sample tomato extract agree with theoretical values,
indicating interference from matrix is minimal.
Spike
Sample Amount
Sample Amount
Spike Amount
Recovery
Analyte
Quantified
Quantified
Theoretical
(%)
1. tetrachloro-
m
-xylene
0
14.4
16
90
2.
α
-BHC
0
7.8
8
98
3.
β
-BHC
0
7.1
8
89
4.
γ
-BHC
0
6.8
8
85
5.
δ
-BHC
0
6.5
8
81
6. heptachlor
0
9.3
8
116
7. aldrin
0
7.2
8
90
8. heptachlor epoxide
0
9.3
8
116
9.
γ
-chlordane
0
6.9
8
86
10. endosulfan I
9.5
18.7
8
115
11.
α
-chlordane
0
7.5
8
94
12. dieldrin
0
17.4
16
109
13. 4,4'-DDE
0
16.8
16
105
14. endrin
0
14.2
16
89
15. endosulfan II
15.4
27.9
16
78
16. 4,4'-DDD
0
13.9
16
87
17. endrin aldehyde
0
12.8
16
80
18. endosulfan sulfate
13.1
28.2
16
94
19. 4,4'-DDT
0
15.7
16
98
20. endrin ketone
0
13
16
81
21. methoxychlor
0
76.8
80
96
22. decachlorobiphenyl
0
16.2
16
101
For more information about this application, or
about GCxGC in general, please contact our
Technical Service chemists, or your Restek rep-
resentative.
Reference
1. Dimandja, J., Anal. Chem. 76 (9): 167A - 174A (2004).
Acknowledgement
This investigation was conducted in collaboration with Jack
Cochran, Director of Separation Science, LECO Corporation,
Las Vegas, NV.
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®
-5 Column (fused silica)
(Crossbond
®
5% diphenyl/95% dimethyl polysiloxane)
Temp. limits: -60 to 325/340°C
ID
df (µm)
length
cat. #
price
0.18mm 0.20 10-Meter
40201
$220
aldrin
8µg/mL
α
-BHC
8
β
-BHC
8
δ
-BHC
8
γ
-BHC (lindane)
8
a-chlordane
8
γ
-chlordane
8
4,4'-DDD
16
4,4'-DDE
16
4,4'-DDT
16
dieldrin
16
endosulfan I
8
endosulfan II
16
endosulfan sulfate
16
endrin
16
endrin aldehyde
16
endrin ketone
16
heptachlor
8
heptachlor epoxide (B)
8
methoxychlor
80
Organochlorine Pesticide Mix AB #2
In hexane:toluene (1:1), 1mL/ampul
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$33
decachlorobiphenyl
2,4,5,6-tetrachloro-
m
-xylene
Pesticide Surrogate Mix
200µg/mL each in acetone, 1mL/ampul
cat. # 32000 (ea.)
$19
Rtx
®
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(Crossbond
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trifluoropropylmethyl polysiloxane)
Temp. limits: -20 to 310/330°C
ID
df (µm)
length
cat. #
price
0.18mm 0.20
10-Meter
45001
$240