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RESTEK Advantage
800-356-1688
Highly Base-Deactivated HPLC
Columns from Restek
Pinnacle™ DB Columns: the Performance
of Popular BDS Columns, with
Plus 1™ Quality and Service
✔ Performance-equivalent replacement for Hypersil ® BDS columns.
✔ In stock, ready to ship.
✔ Stock columns in analytical to prep scale.
By Greg France, HPLC Products Marketing Manager, and Vernon Bartlett, HPLC Innovations Team Manager
When we set out to create a new base-deactivated sili-
ca, we had five specific goals:
- A material that would match or exceed the per-
formance of any base-deactivated “Type B” silica.
- A material that would provide chromatographic
separations equivalent to Hypersil® BDS silica.
- A material with low metals content, for sharp,
symmetric peaks for basic analytes.
- A material capable of long lifetime in challenging
HPLC environments.
- An effective and efficient manufacturing process,
to ensure columns are always available.
Pinnacle™ DB silica meets all of these goals.
Pinnacle™ DB silica performs as well as any base-
deactivated Type B silica, or better, and closely
matches the desirable physical characteristics of
Hypersil ® BDS silica. For certain physical parame-
ters we intentionally deviated from Hypersil ® BDS
material — total metals content, for example.
Fewer metal ions on the surface of Pinnacle ™ DB
silica particles ensure sharper and more symmetric
peaks for basic analytes.
Of course, what really matters is how separations on
columns made with the two silicas compare. Figure
1 pairs chromatograms for a neutral/base test mix
from a C18 bonded phase Pinnacle™ DB column
and a Hypersil® BDS column, obtained using the
same instrument, mobile phase, and conditions.
Retention, peak shape, and efficiency are nearly
identical in the two chromatograms.
If physical data and chromatographic comparisons
show Pinnacle™ DB and Hypersil® BDS materials are
very closely matched, why use Pinnacle ™ DB columns?
In brief: competitive prices, fast delivery, and unsur-
passed Restek service. Why settle for less? But, we
want you to be completely comfortable in evaluating
our columns. With that in mind, we guarantee sepa-
rations of your samples on Pinnacle™ DB columns will
be comparable to separations on Hypersil® BDS
columns. If a Pinnacle™ DB column does not meet
your satisfaction, simply send us copies of chro-
matograms for your application on the Pinnacle ™ DB
column and on a Hypersil ® BDS column. We will
credit your account for the price of the Pinnacle ™ DB
column, and you can keep the column - free!
The Pinnacle™ DB line currently includes C18, C8,
and cyano bonded phases, and bare silica. Example
applications for each of the bonded phase columns,
including fast LC analysis, and Pinnacle ™ DB /
Hypersil ® BDS comparisons, are shown in Pinnacle™
DB HPLC Columns as Replacements for Hypersil®
BDS, Restek lit. cat.# 59742, available on request.
If you need a second source for Hypersil® BDS col-
umn performance, if you want a rugged, high quality,
base-deactivated material, or if you simply are looking
for a reliable supplier who provides columns quickly,
Pinnacle™ DB columns are your answer. Of course, as
always, you'll get Plus 1 ™ service and prompt, expert
technical help when you deal with Restek.
Figure 1- Pinnacle™ DB columns and Hypersil® BDS columns provide nearly identical
retention, peak symmetry, and efficiency.
LC_0264
LC_0263
Hypersil® BDS C18 Column
Pinnacle™ DB C18 Column Retention Time (min.)
Peak List Conc. (µg/mL) Pinnacle™ DB Hypersil® BDS
1. uracil 50 1.676 1.706
2. aniline 750 2.077 2.127
3. 2-nitroaniline 350 2.618 2.687
4. 2,4-dinitroaniline 350 2.909 2.970
5. 3,5-xylenol 2500 3.668 3.804
6. anisole 2500 4.181 4.359
7. toluene 3500 6.979 7.383
Column: Pinnacle™ DB C18 Hypersil® BDS C18
cat.#: 9414565 25105-154630*
Dimensions: 150 x 4.6mm 150 x 4.6mm
Particle Size: 5µm 5µm
Pore Size: 140Å 130Å
Conditions:
Mobile Phase: 50mM KH2 PO4, pH 3.5:methanol (35:65 v/v)
Flow: 1.0mL/min.
Temp.: ambient
Det.: UV @ 254nm
Sample:
Inj.: 2µL
Sample Diluent: methanol:water (50:50 v/v)
Sample Temp.: ambient
*Hypersil catalog number; column not available from Restek.
Pinnacle™ DB C18 Column (USP L1)
(5µm silica, 4.6mm ID)
Length cat.#
150mm 9414565
HPLC Column Selection Guide
(lit. cat.# 59454A)
A useful chart to keep with your workbooks, or
post on a wall.
Quickly scan
important charac-
teristics of Restek
HPLC columns.
Includes brief,
practical guide-
lines for choos-
ing stationary
phase, particle
size, pore
diameter, and
column
dimensions.
For the complete selection of
Pinnacle™ DB Columns, visit our website,
,
or request Applications Note 59742.
More information and additional
Pinnacle™ DB / Hypersil® BDS
comparisons are included in
Applications Note 59742
(free on request).