214 / 298
With these precautions observed, fast LC can be an
excellent time- and money-saving tool for many ana-
lysts and many applications. If you would like to dis-
cuss whether this approach is suitable for your
application, please contact our Technical Service
Group; they are ready to help you.
• 9 •
www.restekcorp.com2003
Volume 2
800-356-1688
Peak List:
Ret. Time (min.)
1. vanillin
2.00
1. vanillin
2.00
2. ethyl vanillin 4.20
Column:
Pinnacle
TM
DB C18
Catalog #:
9414555
Dimensions:
50 x 4.6mm
Particle Size:
5µm
Pore Size:
140Å
Conditions:
Mobile Phase:
A: 0.2% v/v conc. phosphoric
acid in water
B: methanol
Isocratic: 80%A: 20%B
Flow:
2mL/min
Temp.:
27°C
Det.:
UV @ 254nm
LC_0252
Sample:
Inj.:
5µL
Conc.:
unknown
Sample Diluent:
20% v/v
commercial
extract in
water
Figure 4
— Fast, highly selective analysis of vanillin/ethyl vanillin
and vanilla bean extract on a Pinnacle
TM
DB C18 column.
Sample:
Inj.:
5µL
Conc.:
200µg/mL each component
Sample Diluent: 4% v/v ethanol in water
LC_0251
Figure 3
— LC/MS analysis of carbamates in less than 20 minutes,
using an Ultra Carbamate column.
Column:
Ultra Carbamate
Catalog #:
9177315
Dimensions:
100 x 4.6mm
Particle Size:
3µm
Pore Size:
100Å
Inj.:
10µL
Sample:
531.1 Carbamate Pesticide Calibration
Mix (cat.# 32273)
Conc.:
100µg/mL each
Solvent:
methanol
Mobile Phase: A: 90:10 water:methanol + 10mM
ammonium formate
B: 10:90 acetonitrile:methanol + 10mM
ammonium formate
Gradient:
90:10 A:B to 10:90 A:B
from 0-15 min.
Flow:
1mL/min. (0.75mL/min. to
UV, 0.25mL/min. to MS)
Temp.:
ambient
MS Conditions:
Det.:
Micromass ZMD
Mode:
ESI+
Capillary V:
3.50
Extractor:
4.0
Ion Energy:
0.4
Multiplier:
650
Source Temp.:
100ºC
Desolv. Temp.:
250ºC
Gas Flow:
490 L/hr.
Compound
Ion
Cone V
1. aldicarb sulfone
223.3
25V
2. aldicarb sulfoxide
207.3
18V
3. oxamyl
237.2*
10V
4. methomyl
163.2
15V
5. 3-hydroxycarbofuran 238.3
15V
6. aldicarb
191.2
8V
7. propoxur
210.2
18V
8. carbofuran
222.3
22V
9. carbaryl
202.2
18V
10. methiocarb
226.3
19V
*Ammonium adduct; others [M+H]+ ions.
Table 1
— Chromatographic conditions
for LC/MS analysis of carbamates.
Four Fast LC Cartridges (Ultra C18, Ultra Aqueous C18, Ultra Cyano, Ultra PFP), Fast LC Cartridge Holder.
Fast LC Development Kits
Description
qty.
cat.#
Fast LC Development Kit—30 x 2.1mm
kit
25296
Fast LC Development Kit—30 x 4.0mm
kit
25297
3µm Fast LC Cartridges
Description
Length
2.1mm ID
4.0mm ID
Ultra C18 Fast LC Cartridge
30mm
91743320
91743340
Ultra Aqueous C18 Fast LC Cartridge
30mm
91783320
91783340
Ultra Cyano Fast LC Cartridge
30mm
91063320
91063340
Ultra PFP Fast LC Cartridge
30mm
91763320
91763340
Fast LC Cartridge Holder
Description
qty.
cat.#
Fast LC Cartridge Holder
ea.
25298
Request our 2003 HPLC minicatalog
Lit. Cat.# 59241A
Conditions listed in Table I
Additional HPLC Columns
Description
Dimensions
cat.#
Ultra Carbamate, 3µm
50mm x 4.6mm ID
9177355
Ultra Carbamate, 3µm
100mm x 4.6mm ID
9177315
Pinnacle
™
DB C18, 5µm
50mm x 4.6mm ID
9414555