ADV01-05_1-298

the

in this issue

Leak Detective

™

…pg. 5

ShinCarbon ST Micropacked Columns

for Permanent Gases

…pg. 6

Optimized Analysis for GC/MS

Screening for Phenols

…pg. 7

New Analytical Reference Materials

…pg. 8–9

Silcosteel

-CR Tubing and Fittings

…pg. 10

Pinnacle

™

PAH HPLC Columns

…pg. 11

Rt-2560 GC Columns and Reference

Materials for FAME Analysis

…pg. 12

Drilled Uniliner

GC Inlet Liner

…pg. 13

Peak Performers

…pg. 14

Cool Tools

…pg. 15

Mini Air Sampling Canisters

…pg. 15

Behind the Scenes

…pg. 16

www.restekcorp.com

2003

vol. 1

Advantage

Pinnacle

™

HPLC Columns

…pg. 1–2

EZ No-Vent

™

GC/MS

Connector

…pg. 3

Optimized Analysis for

Screening for Semivolatile

Organic Compounds

…pg. 4–5

Innovators of High Resolution

Chromatography Products

by Greg France, HPLC Products Marketing Manager

New Pinnacle

™

DB HPLC Columns

Superior Performance for Basic Compounds

✔

Silica manufactured in Restek facilities, for total control of product quality.

✔

Unique manufacturing process ensures sharp, symmetric peaks for basic analytes.

✔

Columns available in narrow bore through preparative-scale formats.

In 2001 our Pinnacle II

™

line of columns put Restek

among the select few HPLC column manufacturers

who manufacture their own silica. We believe that to

truly control HPLC variables, a manufacturer must

control the entire column manufacturing process,

beginning at the initial step—making a well-charac-

terized, consistently-performing silica.

Now, we are pleased to introduce a new line of

HPLC columns, Pinnacle

™

DB columns, prepared

from our newest silica support. Pinnacle

™

DB silica

is a highly base-deactivated silica,

suitable for a wide range of

challenging applications. It

is ideal for analyses of

basic compounds, or

mixtures of varied func-

tionality. Part of our

manufacturing process

for Pinnacle

™

DB silica is

a unique processing step

that creates a base-deactivat-

ed particle with excellent per-

formance characteristics. The base

deactivation and attractive mass transfer capabilities

of the particles enable Pinnacle

™

DB silica to

resolve and elute basic compounds (e.g., pharma-

ceuticals) without severe peak tailing—with mini-

mal or no need for mobile phase modifiers such as

tetrabutylammonium (TBA). Physical characteris-

tics of Pinnacle

™

DB silica are listed in Table I

(page 2).

Column manufacturers often use an analysis of a

pyridine/phenol test mix to demonstrate a column's

separation capabilities and indicate the peak shape

that can be anticipated for basic compounds.

Figure 1 shows pyridine/phenol separations from a

Pinnacle

™

DB C18 and a non-base-deactivated C18

column. The sharp, symmetric peaks from the

Pinnacle

™

DB C18 column—without a mobile

phase modifier—are what an analyst can expect for

many pharmaceutical or other basic analytes.

Figure 2 shows an array of basic pharmaceutical

compounds analyzed, with excellent results, on a

Pinnacle

™

DB C18 column. Note the consistent

peak symmetry. Often, neutral or acidic compounds

can be present with basic pharmaceutical com-

pounds as impurities or degradation products.

Pinnacle

™

DB columns are highly suited to these

challenges as well. Along with the monomeric C18

bonded phase, the Pinnacle

™

DB line currently

Figure 1

Pinnacle

™

DB C18 columns provide sharp, symmetric peaks for basic analytes, without a

mobile phase modifier.

LC_0247

Peak List:

Conc.

1. uracil

5.0µg/mL

2. pyridine

0.1µL/mL

3. phenol

1.86mg/mL

Sample:

Inj.:

5.0µL

Sample Diluent:

mobile phase

Conditions:

Mobile Phase:

A: 20mM potassium

phosphate, pH 7.0

B: acetonitrile

Isocratic: 80%A:20%B

Flow:

1.0mL/min

Temp.:

ambient

Det.:

UV @ 254nm

Column:

Pinnacle

™

DB C18

Catalog #:

9414565

Dimensions:

150 x 4.6mm

Particle Size:

5µm

Pore Size:

140Å

Column:

Pinnacle

™

C18

Catalog #:

9214565

Dimensions:

150 x 4.6mm

Particle Size:

5µm

Pore Size:

110Å

LC_0248