147 / 298
• 5 •
Figure 3
A Pinnacle
II
™
Silica column effectively sepa-
rates the positional isomers of tocopherol
by normal phase HPLC.
Figure 2
Phenolic antioxidants can be quantitated easily using a Pinnacle
II
™
C18 column and UV
detection at 280nm.
LC_0198
1
2
3
4
0
2
4
6
8
10
12
14
16
18min.
Peak List:
conc.:
(ppm)
1. propyl gallate
168
2. TBHQ
182
3. 2-BHA + 3-BHA 197
4. BHT
193
Sample:
Inj.:
10µL
Conc.:
see peak list
Solvent:
methanol
Column:
Pinnacle II
™
C18
Catalog #:
9214565
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
110Å
Conditions:
Mobile Phase:
A = 1% acetic acid in water
B = methanol
Time A B
(min.) (%) (%)
0
50 50
4
50 50
10 10 90
25 10 90
26 50 50
Flow:
1.0mL/min
Temp.:
30°C
Det.:
UV @ 280nm
1
2
3
4
0
2
4
6
8
10
12
14 min.
LC_0197
Peak List:
1.
α
-tocopherol
2.
β
-tocopherol
3.
γ
-tocopherol
4.
δ
-tocopherol
Column:
Pinnacle II
™
Silica
Catalog #:
9210565
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
110Å
Conditions:
Mobile Phase:
isopropyl alcohol:hexane (0.5:99.5)
Flow:
0.6 mL/min
Temp.:
30°C
Det.:
UV @ 295nm
Pinnacle
II
™
C18 5µm Columns
Pinnacle
II
™
Silica 5µm Columns
Ultra Aqueous C18 5µm Columns
1.0mm ID
2.1mm ID
3.2mm ID
4.6mm ID
Length
cat.#
cat.#
cat.#
cat.#
price
30mm
9178531
9178532
9178533
9178535
50mm
9178551
9178552
9178553
9178555
100mm
9178511
9178512
9178513
9178515
150mm
9178561
9178562
9178563
9178565
200mm
9178521
9178522
9178523
9178525
250mm
9178571
9178572
9178573
9178575
1.0mm ID
2.1mm ID
3.2mm ID
4.6mm ID
Length
cat.#
cat.#
cat.#
cat.#
price
30mm
9210531
9210532
9210533
9210535
50mm
9210551
9210552
9210553
9210555
100mm
9210511
9210512
9210513
9210515
150mm
9210561
9210562
9210563
9210565
200mm
9210521
9210522
9210523
9210525
250mm
9210571
9210572
9210573
9210575
1.0mm ID
2.1mm ID
3.2mm ID
4.6mm ID
Length
cat.#
cat.#
cat.#
cat.#
price
30mm
9214531
9214532
9214533
9214535
50mm
9214551
9214552
9214553
9214555
100mm
9214511
9214512
9214513
9214515
150mm
9214561
9214562
9214563
9214565
200mm
9214521
9214522
9214523
9214525
250mm
9214571
9214572
9214573
9214575
XF
Fitting
Guard
Cartridge
XG-XF
Fitting
Cap
Frit
End
Fitting
Trident
™
Integral HPLC Guard Column System
Maximum protection against contaminants and particulate matter.
Trident
™
Direct
HPLC Guard Column System
Three levels of protection!
Trident
™
Direct high-pressure filter
Protection against particulate matter.
Trident
™
Direct 1cm guard cartridge
holder with filter
Moderate protection against particulate matter and
irreversibly adsorbed compounds.
Trident
™
Direct 2cm guard cartridge
holder with filter
Maximum protection against particulate matter and
irreversibly adsorbed compounds.
Sample:
Inj.:
10µL
Conc.:
approx. 1.25% soy oil
Solvent: hexane
for
more
info
For additional information about Trident
™
guard columns,
request the Trident
™
Fast Facts (lit. cat.# 59314 and 59896).