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Marine oil products, such as fish oils, are generat-
ing a significant amount of interest based on recent
medical studies indicating health benefits from con-
suming these products. Of these oils, the long-chain
polyunsaturated fatty acids (PUFAs) such as eicos-
apentaenoic acid (EPA, C20:5) and docosa-
hexaenoic acid (DHA, C22:6) have been studied
extensively due to claims that they reduce the risk
by Rebecca E. Wittrig, Ph.D., Food, Flavor, and Fragrance
Innovations Team Manager
Rapid Analysis of Marine
Oil-Based FAMEs
FAMEWAX™ Capillary GC Column
✔ Excellent resolution in less than 10 minutes!
✔ Ideal for all FAME analyses, particularly polyunsaturated FAMEs.
of a variety of diseases including hypertension, car-
diovascular disease, and autoimmune disorders.
These Omega-3 fatty acids are present in high
quantities in fish oils. Because the health benefits
are thought to be associated with these specific
PUFAs, it is necessary to accurately monitor their
levels in marine oil raw materials and finished
products.
30m, 0.32mm, 0.25µm FAMEWAX™ (cat.# 12498)
Oven temp.: 195°C to 240°C at 5°C/min. (hold 1 min.); Inj. & det. temp.: 250°C (inj.), 275°C (det.); Carrier gas:
hydrogen; Linear velocity: 3mL/min. (constant flow); Split ratio: 100:1; Injection volume: 1µL; Std. concentration:
10,000µg/mL in isooctane (total FAMES), see breakdown in peak list.
Figure 1
The FAMEWAX™ column shows excellent resolution of marine oil-based FAMEs
in less than 10 minutes! Note the excellent separation of peaks 18-20.
A typical procedure for analyzing marine oil-based
FAMEs involves saponifying the oils and forming the
methyl ester derivatives. The FAMEs then can be
separated and quantitated by GC using a polar poly-
ethylene glycol (PEG)-type capillary column. In
specific, the Omega-3 fatty acids (EPA, C20:5 and
DHA, C22:6) need to be completely resolved from
all of the other FAME species present. A standard
procedure requires a run time of 30-40 minutes to
achieve this separation. In comparison, the Restek
FAMEWAX™ column shows an excellent separation
in less than 10 minutes! Using the FAMEWAX™ capil-
lary GC column will give you much higher through-
put for your marine oil-based nutraceutical prod-
ucts and ingredients.
Restek offers several different FAME mixtures for
your food and nutraceutical testing needs. Refer to
the annual Chromatography Products Guide
(lit. cat.# 59662) or visit us online at
for the complete listing.
✔ Marine Oil FAME Mix
Contains:
Chain Description % by Weight
C14:0 methyl myristate 6.0
C14:1 methyl myristoleate 1.0
C16:0 methyl palmitate 16.0
C16:1 methyl palmitoleate 5.0
C18:0 methyl stearate 8.0
C18:1 methyl oleate 13.0
C18:1 methyl vaccenate 4.0
C18:2 methyl linoleate 2.0
C18:3 methyl linolenate 2.0
C20:0 methyl arachidate 1.0
C20:1 methyl 11-eicosenoate 9.0
C20:2 methyl 11-14-eicosadienoate 1.0
C20:4 methyl arachidonate 3.0
C20:3 methyl 11-14-17-eicosatrienoate 1.0
C20:5 methyl eicosapentaenoate 10.0
C22:0 methyl behenate 1.0
C22:1 methyl erucate 3.0
C22:6 methyl docosahexaenoate 12.0
C24:0 methyl lingnocerate 1.0
C24:1 methyl nervonate 1.0
Description cat.#
100mg 35066
conc.
1. C14:0 FAME 600µg/mL
2. C14:1 FAME 100
3. C16:0 FAME 1600
4. C16:1 FAME 500
5. C18:0 FAME 800
6. C18:1 FAME (oleate) 1300
7. C18:1 FAME (vaccenate) 400
8. C18:2 FAME 200
9. C18:3 FAME 200
10. C20:0 FAME 100
✔ FAMEWAX™ (fused silica) (Crossbond® polyethylene glycol)
Length (m) ID (mm) df(µm) Temp. Limits cat.#
30 0.25 0.25 20 to 250°C 12497
30 0.32 0.25 20 to 250°C 12498
30 0.53 0.50 20 to 250°C 12499
conc.
11. C20:1 FAME 900
12. C20:2 FAME 100
13. C20:4 FAME 300
14. C20:3 FAME 100
15. C20:5 FAME 1000
16. C22:0 FAME 100
17. C22:1 FAME 300
18. C24:0 FAME 100
19. C22:6 FAME 1200
20. C24:1 FAME 100
GC_FF00499 Monitor Food Packaging?
Check out our new Applications Note
Monitoring Volatile Compounds in Food
Contact Packaging Using Purge and Trap
GC/MS and an Rtx® -5MS Capillary Column
(lit. cat.# 59348).
Watch...
for new food, flavor, and fragrance
reference materials coming soon.