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Figure 1
The Rtx
®
-35 Amine column prevents adsorption and improves response for amines.
Innovators of High Resolution
Chromatography Products
the
in this issue
Marine Oil-Based FAME Analysis Using
FAMEWAX
™
…pg. 6
GC Capillary Guard Columns
…pg. 7
Fast Sim Dist with an MXT
®
-1HT and
the GC Racer
™
System
…pg. 8–9
Siltek
™
Uniliner
®
Liner for Semivolatile
Compound Analysis
…pg. 10–11
Haloacetic Acid Analytical Reference
Materials
…pg. 12
TPH Analytical Reference Materials
for Water Quality Testing
…pg. 13
Fast Petrochemicals Analysis
…pg. 14
Cool Tools
…pg. 15
Behind the Scenes
…pg. 16
800-356-1688
814-353-1300
www.restekcorp.comWinter
’02
by Neil Mosesman, GC Columns Product Marketing Manager
INTERNATIONAL
Intermediate Polarity Capillary
Column for the GC Analysis of
Basic Compounds
Rtx
®
-35 Amine GC Column
Basic
Compound Analyses
Using Rtx
®
35 Amine
…pg. 1–2
InfraRed
™
and Merlin
Microseal
™
Septa
…pg. 3
Degasys In-Line Mobile
Phase Degasser
…pg. 4
Three Generations of
Restek Silica Technology
…pg. 5
Amines and nitrogen hetero-
cyclics are used to manufacture
a wide variety of products
including dyes, chelating
agents, stabilizers, pesticides,
and pharmaceuticals. Analyzing
amines and other basic com-
pounds by gas chromatography
(GC) can be difficult as the
active nature of these com-
pounds causes adsorption and peak tailing. Several
years ago Restek introduced the Rtx
®
-5 Amine col-
umn, which uses a unique deactivation chemistry to
improve the response and peak symmetry of
amines, especially alkyl amines, diamines, tri-
amines, ethanolamines, and ethyleneamines. Now
Restek is introducing the new Rtx
®
-35 Amine col-
✔
Achieves symmetrical peaks for basic compounds.
✔
Improved response over traditional columns.
✔
Resolves low-molecular-weight primary amines.
new
Advantage
umn, which employs the same deactivation technol-
ogy as the Rtx
®
-5 Amine, but features a higher
polarity that is ideal for separating more polar
amines and lower molecular weight amines.
Enhanced Peak Symmetry for Amines
The proprietary deactivation of the Rtx
®
-35 Amine
results in improved response and better peak sym-
metry compared to other 35% phenyl columns
(Figure 1). A test mixture with a wide variety of
amines at concentrations of 10 to 15ng/µL was ana-
lyzed using an Rtx
®
-35 Amine column and a stan-
dard 35% phenyl column. While the Rtx
®
-5 Amine
column shows complete adsorption of
diethylenetriamine and diethanolamine, the
Rtx
®
-35 Amine offers excellent response and peak
shape for these compounds.
30m, 0.53mm ID, 1.0µm Rtx
®
-35Amine (cat.# 11355)
30m, 0.53mm ID, 1.0µm Rtx
®
-35 (cat.# 10455)
Sample:
amine test mix (cat.# 35002);
Conc.:
450-
900ppm;
Solvent:
methanol/methylene chloride;
Sample size:
1.0µL;
Inj. temp.:
250°C;
Inj. mode:
split (10:1);
Inlet liner:
base-deactivated 4mm Single
Gooseneck (cat.# 20798-210.1);
Carrier gas:
helium;
Linear velocity:
30cm/sec.;
Detector:
FID/300°C;
Temp. program:
110°C (hold 4.0 min.) to 200°C @
8°C/min. (hold 5.0 min.)
1. pyridine
2. 1,2-butanediol
3. C10
4. 2-nonanol
5. diethylenetriamine
6. C12
7. diethanolamine
8. 2,6-dimethylaniline
GC_CH00577
GC_EX00579