730
HPLCCHROMATOGRAMS |
FOODS&FLAVORS
Dietary Supplements
LC_0174
0
2
4
6
8 min.
1
Allicin inGarlicTabletsonPinnacle® IIC18
Column:
Pinnacle
®
II C18
Cat.#:
9214565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
110Å
Conditions:
Mobile phase: water:methanol
(50:50 v/v)
Flow:
0.8mL/min.
Temp.:
30°C
Det.:
UV@ 240nm
PhenethylGlucosinolateonUltraAqueousC18
Sample:
Inj.:
10µL
Conc.:
1,000µg/mL
Solvent:
water
Column:
Ultra Aqueous C18
Cat.#:
9178565
Dimensions:
150mm x 4.6mm
Particle size: 5µm
Pore size:
100Å
Conditions:
Mobile phase: A: 50mM potassium
phosphate, pH 2.5
B: acetonitrile
Time (min.): %B
0.0
0
10
75
11
0
16
0
Flow:
1.0mL/min.
Temp.:
ambient
Det.:
UV@ 210nm
Peak List:
1. phenethyl glucosinolate
LC_0166
Sample:
Inj.:
20µL
Conc.:
1.2mg/mL
garlic tablet inwater
Solvent:
deionizedwater
Peak List:
1. allicin
Phenolics in
Echinacea
onPinnacle® IIC18
LC_0195
Column:
Pinnacle
®
II C18
Cat.#:
9214565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
110Å
Conditions:
Mobile phase: A: 0.1% phosphoric acid
B: acetonitrile
Time (min.) %B
0
10
13
22
14
40
14.5 40
15
10
20
10
Flow:
1.5mL/min.
Temp.:
35°C
Det.:
UV@ 330nm
Peak List:
1. caftaric acid
2. chlorogenic acid
3. echinacoside
4. cichoric acid
Sample:
Inj.:
10µL
Conc.:
6.7mg/mL
Echinacea
capsule
contents in sample diluent
Solvent:
ethanol:water (70:30, v/v)
Sample temp.: 25°C
0
2
4
6
8
10
12
14 min.
1
2
4
3
LC_0197
Tocopherols inSoyOilonPinnacle® IISilica
Peak List:
1.
α
-tocopherol
2.
β
-tocopherol
3.
γ
-tocopherol
4.
δ
-tocopherol
Sample:
Inj.:
10µL
Conc.:
approx. 1.25% soy oil
Solvent:
hexane
Column:
Pinnacle
®
II Silica
Cat.#:
9210565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
110Å
Conditions:
Mobile phase:
isopropyl alcohol:hexane
(0.5:99.5)
Flow:
0.6mL/min.
Temp.:
30°C
Det.:
UV@ 295nm
Website :
E-mail :
TelNo : 03 9762 2034 . . . inAUSTRALIA
Mar 2011
