724
HPLCCHROMATOGRAMS |
FOODS&FLAVORS
Drug Residues
Peak List:
Conc. (ppb)
Time (min)
1. d5-AMOZ
0.3
3.7
2. AMOZ
0.3
3.8
3. AHD
0.3
5.46
4. d4-AOZ
0.3
5.49
5. AOZ
0.3
5.51
6. SC
0.3
5.6
Column:
Ultra C18
Cat.#:
9174312
Dimensions:
100mm x 2.1mm
Particle size:
3µm
Pore size:
100Å
Conditions:
Mobile phase:
A: 0.05% formic acid inmethanol
B: 0.05% formic acid – 5mMNH
4
formate inwater
Time (min) %B
0
90
2.5
90
5
10
10
10
12
90
15
90
Flow:
200µL/min.
Temp.:
30°C
Det.:
MS/MS triple quadrupoles (Thermo Scientific Discovery)
Analyzer Parameters:
Ion source:
ESI (electrospray ionization)
Only segment:
15min.
Polarity:
positive
Data type:
centroid
Scanmode:
SRM product
Scanwidth (m/z): 0.7
Scan time (s):
0.25
Peakwidth:
Q1: within 0.7
Q2: 0.7
Collision gas
pressure (mTorr): 1.5 (argon)
Divert valve:
active, with 3 positions
Positions-1° 2min., 2° 8min., 3° 5min.
Analyte
Prec. Ion Prod. Ion Collision E Tube Lens
AOZ
236
134
12 V
120
AMOZ
335
291
10 V
100
SC
209
166
12 V
80
AHD
249
134
12 V
110
AMOZ= 3-amino-5-morpholinomethyl-2-oxazolidinone
AHD= 1-aminohydantoin hydrochloride
AOZ= 3-amino-2-oxazolidinone
SC= semicarbazide
NitrofuranMetabolitesonUltraC18 (LC/MS/MS)
LC_0323
d5-AMOZ
AMOZ
AHD
d4-AOZ
AOZ
SC
for
more
info
Visit
toviewour "
AnalysisofNitrofurans
inHoneyUsingLC/MS/MSandanUltraC18Column
" article.
Website :
E-mail :
TelNo : 03 9762 2034 . . . inAUSTRALIA
Mar 2011
