717
HPLCCHROMATOGRAMS |
ENVIRONMENTAL
Polycyclic Aromatic Hydrocarbons
Peak List:
Retention Time
1. naphthalene
6.19
2. acenaphthylene
7.16
3. 1-methylnaphthalene
7.78
4. 2-methylnaphthalene
8.17
5. acenaphthene
8.46
6. fluorene
8.82
7. phenanthrene
9.64
8. anthracene
10.39
9. fluoranthene
11.22
10. pyrene
11.75
11. benzo(a)anthracene 13.55
12. chrysene
13.91
13. benzo(j)fluoranthene 14.92
14. benzo(b)fluoranthene 15.27
15. benzo(k)fluoranthene 15.82
16. benzo(a)pyrene
16.35
17. dibenzo(a,h)anthracene 17.10
18. benzo(ghi)perylene
17.67
19. indeno(1,2,3-cd)pyrene 17.99
PolycyclicAromaticHydrocarbonsonPinnacle® IIPAH
EPA8310–18componentstandard+benzo(j)fluoranthene
Sample:
Inj.:
10µL
Conc.:
20µg/mL each component
Sample diluent: acetonitrile
Column:
Pinnacle
®
II PAH
Cat.#:
9219463
Dimensions:
150mm x 3.2mm
Particle size:
4µm
Pore size:
110Å
Conditions:
Instrument:
Shimadzu ProminenceHPLC
Mobile phase:
A: purifiedwater
B: acetonitrile
Time (min.)
%B
0
40
7
60
16
100
18.9
100
19
40
Flow:
1.2mL/min.
Temp.:
30°C
Det.:
UV@ 254nm
LC_EV0449
NEW!
PolycyclicAromaticHydrocarbons
Pinnacle® IIPAH
1. naphthalene
2. acenaphthylene
3. acenaphthene
4. fluorene
5. phenanthrene
6. anthracene
7. fluoranthene
8. pyrene
9. benzo(a)anthracene
10. chrysene
11. benzo(b)fluoranthene
12. benzo(k)fluoranthene
13. benzo(a)pyrene
14. benzo(ghi)perylene
15. dibenzo(a,h)anthracene
16. indeno(1,2,3-cd)pyrene
* contaminant
Sample:
Inj.:
10µL
Conc.:
10µg/mL each component
Sample diluent: methanol
Column:
Pinnacle
®
II PAH
Cat.#:
9219463
Dimensions:
150mm x 3.2mm
Particle size:
4µm
Pore size:
110Å
Conditions:
Instrument:
Shimadzu Prominence
Mobile phase:
A: water
B: methanol
Time (min.) %B
0.0
75
10.0
90
22.0
100
Flow:
1.0mL/min.
Temp.:
30°C
Det.:
UV@ 254nm
LC_EV0480
0
2
4
6
8
10
12
14
16
18
20
22
24
1
2
*
3
4
5
6
7
8
9
10
12
11
13
14
15
16
Baseline resolutionofPAHs
inmethanol!
Website :
E-mail :
TelNo : 03 9762 2034 . . . inAUSTRALIA
Mar 2011
