721
HPLCCHROMATOGRAMS |
FOODS&FLAVORS
Food Contaminants
Sample:
multicomponent pesticide standard
Inj.:
10µL
Conc.:
1ppb each pesticide
Sample diluent:
acetonitrile
Column:
Ultra Aqueous C18
Cat.#:
9178312
Dimensions:
100mm x 2.1mm
Particle size:
3µm
Pore size:
100Å
Conditions:
Instrument:
Shimadzu Prominence
®
UFLC
XR
Mobile phase:
A: 10mMNH
4
OAc inwater
B: 10mMNH
4
OAc inmethanol
Time (min.)
%B
0.0
20
8.0
90
12.0
100
14.8
100
14.9
20
Flow:
500µL/min
Temp.:
35°C
Det.:
Applied Biosystems 4000QTRAP
®
LC/MS/MS system
Ion Source:
TurboIonSpray
®
A& C: ESI+
B: ESI-
IonSpray Voltage: 5kV (ESI+), -4.2kV (ESI-)
Gas 1:
50psi
Gas 2:
60psi
SourceTemp.:
600°C
Pesticideson
UltraAqueousC18 (LC/MS/MS)
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
11.0
12.0
13.0
14.0
15.0
Time (min.)
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
11.0
12.0
13.0
14.0
15.0
Time,min
0.0
1.0
2.0
3.0
4.0
5.0
6.0
7.0
8.0
9.0
10.0
11.0
12.0
13.0
14.0
Time (min.)
0.0
A: Pesticides in positive ionmode
B: Pesticides in negative ionmode
C: Improved retention of difficult
pesticides in positive ionmode
LC_FF0490A
LC_FF0490
LC_FF0490B
methamidophos
acephate
omethoate
propamocarb
NEW!
Visit
to
viewour "
ComprehensivePesticide
ResidueAnalysisbyLC/MS/MSUsingan
UltraAqueousC18Column
" article
(contains compound identifications
andMSdetails).
for
more
info
Website :
E-mail :
TelNo : 03 9762 2034 . . . inAUSTRALIA
Mar 2011
