725
HPLCCHROMATOGRAMS |
FOODS&FLAVORS
Drug Residues
Sample:
Inj.:
3µL
Conc.:
25µg/mL each component
Sample diluent: methanol
Column:
Ultra II
®
Biphenyl
Cat.#:
9609853
Dimensions:
50mm x 3.0mm
Particle size:
2.2µm
Pore size:
100Å
Conditions:
Instrument:
Shimadzu ProminenceUFLC
XR
Mobile phase:
0.1% formic acid
inwater:methanol (75:25)
Time (min.) %B
0
25
3.5
65
Flow:
1.0mL/min.
Temp.:
30°C
Det.:
UV@ 254nm
Peak List
Ret. Time (min.)
1. sulfadiazine
1.496
2 sulfathiazole
1.663
3. sulfamerazine
1.997
4. sulfamethoxazole
2.381
5. sulfamethazine
2.523
6. sulfachlorpyridizine
2.651
7. sulfamethoxine
3.228
SulfaDrugs
Ultra II®Biphenyl
LC_PH0489
NEW!
Sample:
milk spiked at 200ppbwith sulfonamides, extractedwithQ-sep™Q110QuEChERS
extraction tube (cat.# 26213), and cleaned upwithQ-sep™Q211QuEChERS dSPE
clean-up tube (cat.# 26216), then concentrated.
Inj.:
10µL
Conc.:
4µg/mL
Sample diluent: 0.2% formic acid
Column:
Pinnacle
®
DBBiphenyl
Cat.#:
9409312
Dimensions:
100mm x 2.1mm
Particle size:
3µm
Pore size:
140Å
Conditions:
Instrument:
Agilent 1100with LECOUnique
®
LC-TOFMS
Mobile phase:
A: 0.2% formic acid
B: methanol:acetonitrile (80:20), with 0.2% formic acid
Time (min.)
%B
0
15
20
100
30
100
Flow:
1mL/min. with preinjection split delivering 0.2mL/min. to column
Temp.:
ambient
Det.:
LECOUnique
®
LC-TOFMS
Interface:
ESI
Ionmode:
positive
Temp.:
120°C
Capillary:
3.8kV
Cone:
50V
Desolvation gas: 4L/min.
Collected:
200-1,000m/z
Compound
[M+H]
+
1. sulfadiazine
251
2. sulfathiazole
256
3. sulfamerazine
265
4. sulfamethazine
279
5. sulfamethoxypyridazine
281
6. sulfamethoxazole
254
7. sulfachloropyridazine
285
8. sulfadimethoxine
311
9. sulfaquinoxaline
301
Sulfonamides inMilkonPinnacle®DBBiphenyl (LC-TOFMS)
LC_FF0502
NEW!
Website :
E-mail :
TelNo : 03 9762 2034 . . . inAUSTRALIA
Mar 2011
