751
HPLCCHROMATOGRAMS |
CLINICAL/FORENSIC
Benzodiazepines
BenzodiazepinesonAllure®PFPPropyl (LC/MS/MS)
Sample:
benzodiazepines
Inj.:
20µL
Conc.:
NA
Solvent:
NA
Column:
Allure
®
PFPPropyl
Cat.#:
9169552
Dimensions:
50mm x 2.1mm
Particle size: 5µm
Pore size:
60Å
Conditions:
Instrument:
Shimadzu ProminenceHPLC
Mobile phase: A: 0.1% formic acid and 1mM
ammonium formate inwater
B: 0.1% formic acid and 1mM
ammonium formate in acetonitrile
Time
Flow %B
(min.)
(µL/min.)
0.0
500
10
10.00
1000
90
15.00
1000
90
15.50
500
10
17.50
500
10
Flow:
see gradient table
Temp.:
40°C
Det.:
Applied Biosystems/MDS Sciex
API 3200™MS/MS system
Ion Source:
Electrospray, positive
IonSpray
Voltage:
NA
Gas 1:
NA
Gas 2:
NA
Source
Temp.:
500°C
Data courtesy of: Applied BiosystemsMDS Sciex
LC_PH0463
Analyze27
benzodiazepines
in less than
10minutes!
CompoundName
Retention Time Precursor Ion
MRM 1 MRM 2
DP
CE
CE
LOQ
(min.)
(amu)
(amu)
(amu)
(MRM 1) (MRM 2) (ng/mL)
7-aminonitrazepam
3.2
252.1
121.1
94.0
51
35
53
1.0
7-aminoclonazepam
3.3
286.1
121.0
222.2
46
41
35
0.5
7-aminoflunitrazepam
3.8
284.1
135.1
226.0
51
39
49
0.5
Bromazepam
3.8
316.0/318.0
182.1
182.1
51
45
45
5.0
α
-hydroxyalprazolam
4.1
325.1
297.2
204.9
51
31
59
2.0
α
-hydroxytriazolam
4.1
359.0
239.2
176.0
61
63
37
5.0
Oxazepam
4.2
287.0
241.1
268.9
41
27
19
10.0
Lorazepam
4.3
321.0/323.1
275.0
277.0
41
31
27
5.0
Estazolam
4.4
295.0
205.0
267.1
51
53
31
2.0
Zaleplon
4.4
306.2
236.3
264.2
56
35
27
0.5
2-hydroxyethylflurazepam 4.5
333.1
211.2
109.0
56
51
41
1.0
Desmethylflunitrazepam 4.5
300.1
254.2
198.2
56
35
51
2.0
Nitrazepam
4.6
282.0
236.1
180.2
71
35
51
2.0
Clonazepam
4.7
316.0
270.2
214.0
56
41
51
2.0
Desalkylflurazepam
4.7
289.1
140.1
226.1
71
41
39
2.0
Temazepam
4.7
301.1/303.1
255.1
257.2
35
30
30
5.0
Triazolam
4.7
343.0
238.9
314.9
61
53
37
1.0
Alprazolam
4.8
309.1
205.1
281.1
56
53
35
1.0
Lormetazepam
4.8
335.0/337.1
289.0
291.1
41
29
29
2.0
Clobazam
4.9
301.1
259.1
224.3
46
29
47
1.0
Flunitrazepam
5.0
314.0
268.1
239.1
56
35
49
1.0
Nordiazepam
5.0
271.1
140.2
164.9
46
37
35
2.0
Zolpiclone
5.4
389.1
244.8
217.0
16
25
41
1.0
D5-Diazepam
5.4
290.1
198.2
-
55
41
-
Diazepam
5.5
285.0
193.2
154.1
55
41
37
1.0
Chlordiazepoxide
6.0
300.1
227.1
283.2
36
31
21
5.0
Prazepam
6.1
325.1
271.1
140.0
81
31
53
2.0
Zolpidem
7.4
308.1
235.1
236.1
56
39
35
0.2
Midazolam
7.9
326.1
291.3
222.0
56
33
63
0.5
Flurazepam
8.5
388.2
315.1
317.1
36
27
27
0.1
Medazepam
9.0
271.0
91.1
207.3
46
41
39
2.0
D3-Doxepine
9.1
283.0
107.1
-
41
35
-
MRMTransitions, retention times,
andLOQvalues.
Bar color indicates shared precursor ions.
Note compoundswith shared precursor ions
are baseline resolved on the Allure
®
PFP
Propyl column, as shown by retention time
comparison.
Data courtesy of Applied BiosystemsMDS Sciex.
Website :
E-mail :
TelNo : 03 9762 2034 . . . inAUSTRALIA
Mar 2011
