744
HPLCCHROMATOGRAMS |
BIOCHEMICAL/CLINICAL
Nucleotides, Nucleosides, Nucleic Acid Bases
0
1
2
3
4
5
6
7
8
9 min.
LC_0137
Sample:
Inj.:
1.0µL
Conc.
100µg/mL each
Solvent:
20mM ammonium acetate,
pH 5.0
Note: 1-3 drops of ammonium
hydroxide added to keep guanine
in solution
Column:
Allure
®
PFPPropyl
Cat.#:
9169565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
60Å
Conditions:
Mobile phase:
20mM ammonium
acetate, pH 5.0:
methanol (85:15, v/v)
Flow:
1.0mL/min.
Temp.:
ambient
Det.:
UV@ 260nm
NucleicAcidBasesonAllure®PFPPropyl
1
2
3
4
5
Peak List:
1. cytosine
2. uracil
3. guanine
4. thymine
5. adenine
Nucleotides,Nucleosides, and
NitrogenousBasesonUltra IBD
Peak List:
Conc. (µg/mL)
1. CDP
146
2. CMP
113
3. ATP
117
4. ADP
145
5. cytidine
155
6. TMP
157
7. AMP
125
8. guanine
36
9. guanosine
103
10. adenine
38
11. adenosine
115
0
2
4
6
8
10 12 14 16 18 min.
6 7
8
9
10
11
1
2
3
4
5
LC_0128
Column:
Ultra IBD
Cat.#:
9175565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
20mM ammonium
acetate, pH 5.8:methanol
(97.5:2.5)
Flow:
1.0mL/min.
Temp.:
35°C
Det.:
UV@ 260nm
Sample:
Inj.:
20µL
Solvent:
20mM ammonium
acetate, pH 5.8
Uracil,Uridine,UTP,UDP, andUMPonUltra IBD
Peak List:
Conc. (µg/mL)
1. UTP
360
2. UDP
360
3. UMP
284
4. uracil
90
5. uridine
230
Sample:
Inj.:
20µL
Solvent:
20mM ammonium
acetate, pH 5.8
Column:
Ultra IBD
Cat.#:
9175565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
20mM ammonium
acetate, pH 5.8:
methanol (97.5:2.5)
Flow:
1.0mL/min.
Temp.:
35°C
Det.:
UV@ 260nm
LC_0133
0
2
4
6 min.
1
2
3
4
5
Thymine,Thymidine, TTP,TDP,
andTMPonUltra IBD
Peak List: Conc. (µg/mL)
1. TTP
224
2. TDP
152
3. TMP
346
4. thymine
88
5. thymidine
318
Sample:
Inj.:
20µL
Solvent:
20mM ammonium
acetate, pH 5.8
Column:
Ultra IBD
Cat.#:
9175565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
20mM ammonium
acetate, pH 5.8:
methanol (97.5:2.5)
Flow:
1.0mL/min.
Temp.:
35°C
Det.:
UV@ 260nm
LC_0132
0
2
4
6
8
10
12
14
16 min.
1
2
3
4
5
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E-mail :
TelNo : 03 9762 2034 . . . inAUSTRALIA
Mar 2011
