745
HPLCCHROMATOGRAMS |
BIOCHEMICAL/CLINICAL
Catecholamines, Purines/Pyrimidines, Amino Acids, Peptides
0
5
10
15
20
25 min.
Purines/PyrimidinesonUltraPFP
Column:
Ultra PFP
Cat.#:
9176575
Dimensions:
250mm x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phase:
0.001% formic acid inwater:
methanol (50:50, v/v)
Flow:
1.0mL/min.
Temp.:
ambient
Det.:
UV@ 260nm
1
2
3
4
5
Peak List:
1. cytosine
2. 5-methylcytosine
3. uracil
4. uric acid
5. hypoxanthine
6. xanthine
7. thymine
8. purine
9. theobromine
10. ethyluracil
11. 6-methylpurine
12. 1,7-dimethylxanthine
13. theophylline
14. propyluracil
Sample:
Inj.:
10µL
Conc.:
100µg/mL each
Solvent:
mobile phase
6
7
8
11
9 10
12
14
13
LC_0136
Peak List:
Conc. (mg/mL)
1. tyrosine
0.2
2. phenylalanine 1.6
3. tryptophan
0.4
Sample:
Inj.:
20µL
Solvent:
mobile phase A
AromaticAminoAcidsonUltraAqueousC18
Column:
Ultra Aqueous C18
Cat.#:
9178565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
100Å
Conditions:
Mobile phases: A: 50mM potassium
phosphate, pH 2.5
B: acetonitrile
5% - 20%B: 0-5min.
20% - 5%B: 5-6min.
Hold at 5%B: 6-13min.
Flow:
1.0mL/min.
Temp.:
30°C
Det.:
UV@ 254nm
0
2
4
6
8
10 min.
LC_0146
1
2
3
0.0
2.0
4.0
6.0
8.0
10.0 12.0 14.0 16.0 18.0 20.0
1
2
3
4 5
6
7
mfr. H
mfr. D
mfr. C
mfr. F
Viva
*
Sample:
Inj.:
20µL peptide test mix
Column:
Viva C18
Cat.#:
9514562
Dimensions:
150mm x 2.1mm
Particle size:
5µm
Pore size:
300Å
Conditions:
Mobilephase:
A: 0.08% trifluoroacetic acid inwater (pH 2.0)
B: 0.08%TFA in acetonitrile, 10%B to 40%B in 20min.
Flow:
0.2mL/min.
Det.:
UV@ 214nm
*High levels of tracemetals (~100ppm) in the silica enhance this separation.
1. val-tyr
2. val-tyr-val
3. meth enkephalin
4. val-4-angiotensin III
5. angiotensin II, III
6. leucine enkephalin
7. angiotensin I
PeptideMixonVivaC18
LC_BC0386
CatecholaminesonAllure®PFPPropyl
Peak List
Retention Time (min.)
1. norepinephrine 3.50
2. levodopa
5.43
3. epinephrine
7.27
4. tyrosine
7.77
5. dopamine
11.3
Sample:
Inj.:
10µL
Conc.:
40µg/mL each component
Sample diluent: mobile phase
Sample temp.: ambient
Column:
Allure
®
PFPPropyl
Cat.#:
9169565
Dimensions:
150mm x 4.6mm
Particle size:
5µm
Pore size:
60Å
Conditions:
Mobile phase:
50mM ammonium
phosphate (pH 3.0, adjusted
with formic acid)
Flow:
1mL/min.
Temp.:
35°C
Det.:
UV@ 266nm
LC_BA0349
Website :
E-mail :
TelNo : 03 9762 2034 . . . inAUSTRALIA
Mar 2011
