84 / 628
82
www.restek.comGC COLUMNS |
APPLICATION-SPECIFIC COLUMNS
Foods, Flavors, & Fragrances
Rxi®-35Sil MS Columns
(fused silica)
(midpolarity Crossbond® phase)
• Special selectivity and excellent inertness for substituted polar compounds, such as
drugs, pesticides, herbicides, PCBs, phenols, etc.
• Provides superior separation for cannabinoids.
• Very low-bleed phase for GC-MS analysis.
• Extended temperature range: 50 °C to 340/360 °C.
The higher aromatic content of the Rxi®-35Sil MS column allows for superior sepa-
ration of cannabinoids over traditional 5-type columns. Baseline separation can be
achieved for a comprehensive list of cannabinoids by using a cost-effective 15 m col-
umn and readily available hydrogen carrier gas. The arylene content of the Rxi®-35Sil
MS stationary phase ensures long column lifetime at the high elution temperatures
required for cannabinoids analysis.
Rxi®-35Sil MS Structure
x
Si
O
65%
CH
3
CH
3
Si
O
y
R
O
CH
3
CH
3
Si
CH
3
CH
3
Si
similar phases
DB-35ms, DB-35msUI, VF-35ms, MR2
15-Meter
30-Meter
ID
df
temp. limits
cat.#
price
cat.#
price
0.25 mm 0.25 µm 50 to 340/360 °C
13820
13823
Cannabis Potency Analysis
Cannabinoids on Rxi®-35Sil MS Using Hydrogen Carrier Gas by GC-FID
GC_FS0501
Column
Rxi®-35Sil MS, 15 m, 0.25 mm ID, 0.25 µm (cat.# 13820)
Sample
Cannabinoids standard (cat.# 34014)
Cannabichromene (cat.# 34092)
delta-8-Tetrahydrocannabinol (THC) (cat.# 34090)
Cannabigerol (cat.# 34091)
Injection
Inj. Vol.:
1 µL split (split ratio 50:1)
Liner:
Sky® 4 mm Precision® liner w/wool (cat.# 23305.5)
Inj. Temp.:
250 °C
Split Vent Flow Rate: 125 mL/min
Oven
Oven Temp.:
225 °C (hold 0.1 min) to 330 °C at 35 °C/min (hold 0.9 min)
Carrier Gas
H
2
, constant flow
Flow Rate:
2.5 mL/min
Detector
FID@ 350 °C
Constant Column +
Constant Make-up:
50 mL/min
Make-up Gas Type:
N
2
Hydrogen flow:
40 mL/min
Air flow:
450 mL/min
Data Rate:
20 Hz
Instrument
Agilent/HP6890 GC
Peaks
t
R
(sec)
1. Cannabichromene
83.3
2. Cannabidiol
85.9
3. Delta-8-Tetrahydrocannabinol
94.9
4. Delta-9-Tetrahydrocannabinol
98.4
5. Cannabigerol
100.9
6. Cannabinol
106.7