Restek-2015

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Chromatographic Properties
The innovative Biphenyl is Restek’s most popular LC stationary phase because it
is particularly adept at separating compounds that are hard to resolve or that elute
early on C18 and other phenyl chemistries. As a result, the rugged Raptor™ Biphenyl column
is extremely useful for fast separations in bioanalytical testing applications like drug and me-
tabolite analyses, especially those that require a mass spectrometer (MS). Increasing retention
of early-eluting compounds can limit ionization suppression, and the heightened selectivity
helps eliminate the need for complex mobile phases that are not well-suited for MS detection.
Column Characteristics:
Stationary Phase Category:Phenyl (L11)
Ligand Type: Biphenyl
Particle: 2.7 µm or 5 µm superficially
porous silica (SPP or “core-shell”)
Pore Size:90 Å
Surface Area:150 m2/g (2.7 µm)
or 100 m2/g (5 µm)
Recommended Usage:
pH range: 1.5–8.0
Maximum Temperature: 80 °C
Maximum Pressure: 600 bar / 8,700 psi
(2.7 µm) or 400 bar / 5,800 psi (5 µm)
Raptor™ Biphenyl LC Columns (USP L11)
Si CH3 CH3
O
Biphenyl
D
ispersion H -Bonding
Polarizabilit y CationExchange
USLC® Column Interaction Profile
(See for more information.)
Properties:
• Increased retention for dipolar, unsaturated, or conjugated
solutes.
• Enhanced selectivity when used with methanolic mobile
phase.
• Ideal for increasing sensitivity and selectivity in LC-MS
analyses.
Switch to a Biphenyl when:
• Limited selectivity is observed on a C18.
• You need to increase retention of hydrophilic aromatics.
LC COLUMNS
Raptor™ SPP Columns
0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0
Time (min)
Time (min)
2.0 2.5 3.0 3.5 1.5 1.0
XIC of isobars
Excellent
separation
of isobaric
compounds.
Human Urine Matrix
Components
Analytes separated from
early-eluting matrix.
Pain Panel in Urine on Raptor™ Biphenyl (50 x 3.0 mm) by LC-MS/MS
LC_CF0568
Column Raptor™ Biphenyl (cat.# 9309A5E)
Dimensions: 50 mm x 3.0 mm ID
Particle Size: 2.7 µm
Temp.: 30 °C
Sample
Diluent: Urine:mobile phase A:mobile phase B (17:76:7)
Conc.: 10-100 ng/mL
Inj. Vol.: 10 µL
Mobile Phase
A: Water + 0.1% formic acid
B: Methanol + 0.1% formic acid
Time (min) Flow (mL/min) %A %B
0.00 0.6 90 10
1.50 0.6 55 45
2.50 0.6 0 100
3.70 0.6 0 100
3.71 0.6 90 10
5.00 0.6 90 10
Detector AB SCIEX API 4000™MS/MS
Ion Source: TurboIonSpray®
Ion Mode: ESI+
Instrument API LC-MS/MS
Notes Lorazepam was prepared at 100 ng/mL;
all other analytes are 10 ng/mL.
➲ For compound listing
including isobars, visit
and
search for LC_CF0568.
NEW!
157
Raptor™ Biphenyl
2.1 mm 3.0 mm 4.6 mm
Length cat.# price cat.# price cat.# price
2.7 µm Columns
30 mm 9309A32 9309A3E 9309A35
50 mm 9309A52 9309A5E 9309A55
100 mm 9309A12 9309A1E 9309A15
150 mm 9309A62 9309A6E 9309A65
5 µm Columns
30 mm — 930953E —
50 mm 9309552 930955E 9309555
100 mm 9309512 930951E 9309515
150 mm 9309562 930956E 9309565
250 mm — — 9309575