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www.restek.comCommon Classifications for LC
Columns by Internal Diameter
LC COLUMNS
Tables and Reference
Classification
Internal Diameter
Capillary
<1.0 mm ID
Micro bore
1.0 mm ID
Narrow bore
2.1–3.0 mm ID
Standard bore
3.2–4.6 mm ID
Semi-prep
10–21.2 mm ID
Prep
30–50 mm ID
Optimal Linear Velocities
Optimal flow rate (mL/min)*
Column ID (mm)
1.9 µm dp
3 µm dp
5 µm dp
2.7 µm Raptor™ 5 µm Raptor™
4.6
—
1.5
1.0
1.6
1.0
3.2
—
0.7
0.5
0.8
0.5
3.0
1.1
0.6
0.4
0.7
0.4
2.1
0.5
0.3
0.2
0.3
0.2
1.0
—
0.07
0.05
0.08
0.05
Do we have the reference info for this????
Solvent Miscibility and Solubility
Acetone
Acetonitrile
Dimethyl formamide
Dimethyl sulfoxide
Dioxane
Ethanol
Isopropyl alcohol
Methanol
Tetrahydrofuran
Water
Benzene
Butanol
Carbon tetrachloride
Chloroform
Cyclohexane
1,2-Dichloroethane
Dichloromethane
Ethyl acetate
Ethyl ether
Heptane
Hexane
Isooctane
Methyl tert-butyl ether
Methyl ethyl ketone
Pentane
Toluene
Xylene
Miscible/Water-Miscible Solvents
Miscible Solvents
Immiscible Solvents
Acetone
Acetonitrile
Dimethyl formamide
Dimethyl sulfoxide
Dioxane
Ethanol
Isopropyl alcohol
Methanol
Tetrahydrofuran
Water
Benzene
Butanol
Carbon tetrachloride
Chloroform
Cyclohexane
1,2-Dichloroethane
Dichloromethane
Ethyl acetate
Ethyl ether
Heptane
Hexane
Isooctane
Methyl tert-butyl ether
Methyl ethyl ketone
Pentane
Toluene
Xylene
To convert a pressure, multiply the units in the left-most column by the conversion factors listed in the columns
to the right.
For example:
10 psi x 0.068 = 0.68 atm
10 bar x 29.5300 = 295.300 inches Hg
HPLC Pump Pressure Conversion Table
Pressure
psi
atm
kg/cm
2
torr
kPa
bar
inches Hg
1 psi =
1
0.068
0.0703
51.713
6.8948
0.06895
2.0359
1 atm =
14.696
1
1.0332
760
101.32
1.0133
29.921
1 kg/cm
2
=
14.223
0.967
1
735.5
98.06
0.9806
28.958
1 torr =
0.0193
0.00132
0.00136
1
0.1330
0.00133
0.0394
1 kPa =
0.1450
0.00987
0.0102
7.52
1
0.0100
0.2962
1 bar =
14.5038
0.9869
1.0197
751.88
100
1
29.5300
1 in Hg =
0.49612
0.0334
0.0345
25.400
3.376
0.03376
1
* Optimal flow rates are mobile phase dependent; table above is provided as a guide.