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Column Characteristics:
particle size:
1.9 µm, 3 µm, or
5 µm, spherical
pore size:
140 Å
carbon load:
6%
pH range:
2.5 to 8
temperature limit: 80 °C
USP phase code:
L1
phase category:
modified C18
ligand type:
proprietary polar
modified and
functionally
bonded C18
Aqueous C18
Si
OO
P
LC COLUMNS
Columns for UHPLC
Pinnacle® DB Columns: 1.9, 3, or 5 µm particles; 140 Å pore size
Restek® Pinnacle® DB columns are built for optimal UHPLC performance.
Pinnacle® DB columns are 100%manufactured by Restek in our Bellefonte, Pennsylvania, facility. Because performance begins with the
support, our Pinnacle® DB UHPLC columns start with base-deactivated spherical silica that is optimized for UHPLC stability. From
there, we bond them with a wide variety of phases to give chromatographers a stable and selective column. Get the most out of your
UHPLC system. Combine selectivity and efficiency by using Restek® Pinnacle® DB UHPLC columns.
Pesticides on Pinnacle® DB Aqueous C18 (LC-MS/MS, ESI+)
LC_FF0479A
Column
Pinnacle® DB Aqueous C18
(cat.# 9418252)
Dimensions:
50 mm x 2.1 mm ID
Particle Size:
1.9 µm
Pore Size:
140 Å
Temp.:
35 °C
Sample
multicomponent pesticide
standard
Diluent:
water
Conc.:
33.3 ppb each pesticide
Inj. Vol.:
5 µL
Mobile Phase
A:
10 mM NH
4
OAc in water
B:
10 mM NH
4
OAc in methanol
Time (min) %B
0
10
1
10
8
90
10
90
11
10
Flow:
0.60 mL/min
Max Pressure:
~517 bar
Detector
Applied Biosystems/MDS
Sciex LC-MS/MS
Model #:
4000 QTRAP® LC-MS/MS
system
Ion Source:
TurboIonSpray®
Ion Spray Voltage: 5 kV
Gas 1:
40 psi (275.8 kPa)
Gas 2:
60 psi (413.7 kPa)
Source Temp.:
500 °C
Instrument
Shimadzu UFLC
XR
➲
For compound listing, visit
www.restek.comand
search for LC_FF0479A.
AqueousC18
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USLC® Column Interaction Profile
(See
page 161for more information.)
Aqueous C18
Si
OO
P
Restek-
manufactured
silica
Chromatographic Properties
The Restek® Aqueous C18 is a rugged, reversed-phase column with a well-balanced retention
profile. It can effectively retain more types of solutes than a conventional C18 and is ideal for
multicomponent LC-MS analyses. The general-purpose Aqueous C18 boasts high reproducibil-
ity and compatibility with many mobile phase conditions—even 100% aqueous. And when used
with a gradient, it eliminates the all-too-common issue of multiple compounds eluting near the
column void time.
Pinnacle® DB Aqueous C18 Columns
(USP L1)
2.1 mm ID
3.0 mm ID
4.6 mm ID
Length
cat.#
price
cat.#
price
cat.#
price
1.9 µm Columns
30 mm
9418232
—
—
50 mm
9418252
—
—
100 mm
9418212
—
—
3 µm Columns
30 mm
9418332
941833E
9418335
50 mm
9418352
941835E
9418355
100 mm
9418312
941831E
9418315
150 mm
9418362
941836E
9418365
5 µm Columns
30 mm
9418532
941853E
9418535
50 mm
9418552
941855E
9418555
100 mm
9418512
941851E
9418515
150 mm
9418562
941856E
9418565
200 mm
9418522
941852E
9418525
250 mm
9418572
941857E
9418575