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Figure 2:
A phenyl hexyl column, used under identical conditions, does not provide
adequate retention or selectivity for sulfonamide residue analysis.
Figure 3:
Ultra selective analysis of sulfonamides on a unique Biphenyl column can be used in conjunction with UHPLC for higher
sample throughput.
Figure 1:
Due to their unique selectivity, Biphenyl columns can provide the retention
and separation needed for accurate sulfonamides analysis with simple, MS-friendly
mobile phases.
LC_GN0531
Column:
Ultra Biphenyl (cat.# 9109565); Dimensions: 150 mm x 4.6 mm ID; Particle Size: 5 µm; Pore Size: 100 Å; Temp.: 25 °C;
Sample:
Diluent: 0.1% Formic acid in water; Conc.: 50 µg/mL; Inj. Vol.: 10 µL;
Mobile Phase:
A: 0.1% Formic acid in water, B: 0.1% Formic
acid in acetonitrile;
Gradient (%B):
0 min (10%), 3.0 min (10%), 20.0 min (40%), 21.0 min (40%); Flow: 1.0 mL/min;
Detector:
UV/Vis @
265 nm;
Instrument:
Shimadzu UFLC
XR
.
Peaks
RT (min)
1. Sulfanilamide
4.40
2. Sulfadiazine
10.18
3. Sulfapyridine
10.63
4. Sulfathiazole
10.99
5. Sulfamerazine
11.72
6. Sulfamethazine
12.94
7. Sulfachlorpyridazine 16.08
8. Sulfadoxine
16.42
9. Sulfisoxazole
17.65
10. Sulfadimethoxine
19.47
11. Sulfaquinoxaline
19.86
Strong retention
minimizes matrix
interference for
sulfanilamide.
Unique Biphenyl selectivity
resolves all sulfonamide peaks.
Sulfanilamide is
susceptible to sample
matrix interferences.
Less selectivity results
in coelutions.
For more about the advantages
of USLC® Biphenyl columns, visit
www.restek.com/uslcLC_GN0533
Column:
Waters XSELECT™ CSH Phenyl-Hexyl; Dimensions: 150 mm x 4.6 mm ID; Particle Size: 5 µm; Temp.: 25 °C;
Sample:
Diluent: 0.1%
Formic acid in water; Conc.: 50 µg/mL; Inj. Vol.: 10 µL;
Mobile Phase:
A: 0.1% Formic acid in water, B: 0.1% Formic acid in acetonitrile;
Gradient (%B):
0 min (10%), 3.0 min (10%), 20.0 min (40%), 21.0 min (40%); Flow: 1.0 mL/min;
Detector:
UV/Vis @ 265 nm;
Instrument:
Shimadzu UFLC
XR
.
Peaks
RT (min)
1. Sulfanilamide
3.07
2. Sulfadiazine
7.15
3. Sulfapyridine
7.43
4. Sulfathiazole
7.96
5. Sulfamerazine
8.54
6. Sulfamethazine
8.53
7. Sulfachlorpyridazine
13.49
8. Sulfadoxine
13.63
9. Sulfisoxazole
15.41
10. Sulfadimethoxine
17.22
11. Sulfaquinoxaline
17.45
LC_GN0532
Column:
Pinnacle® DB Biphenyl (cat.# 9409212); Dimensions: 100 mm x 2.1 mm ID; Particle Size: 1.9 µm;
Pore Size: 140 Å; Temp.: 25 °C;
Sample:
Diluent: 0.1% Formic acid in water; Conc.: 50 µg/mL; Inj. Vol.: 2 µL;
Mobile Phase:
A: 0.1% Formic acid in water, B: 0.1% Formic acid in acetonitrile;
Gradient (%B):
0 min
(5%), 8 min (40%); Flow: 0.4 mL/min;
Detector:
UV/Vis @ 265 nm;
Instrument:
Shimadzu UFLC
XR
.
Peaks
RT (min)
1. Sulfanilamide
1.55
2. Sulfadiazine
3.74
3. Sulfapyridine
4.09
4. Sulfathiazole
4.24
5. Sulfamerazine
4.35
6. Sulfamethazine
4.91
7. Sulfachlorpyridazine
5.87
8. Sulfadoxine
5.99
9. Sulfisoxazole
6.37
10. Sulfadimethoxine
7.14
11. Sulfaquinoxaline
7.40
Ultra Biphenyl Columns
(USP L11)
Physical Characteristics:
Description
cat.#
5 µm Columns
150 mm, 4.6 mm ID
9109565
5 µm Columns
150 mm, 4.6 mm ID (with Trident Inlet Fitting)
9109565-700
particle size: 3 µm or 5 µm, spherical
pore size: 100 Å
carbon load: 15%
endcap: fully endcapped
pH range: 2.5 to 8
temperature limit: 80 °C
ordering
note
For guard cartridges for these columns, visit our
website at
www.restek.comPinnacle® DB Biphenyl Columns
(USP L11)
Physical Characteristics:
Description
cat.#
1.9 µm Columns
100 mm, 2.1 mm ID
9409212
particle size: 1.9 µm, 3 µm, or 5 µm,
spherical
pore size: 140 Å
carbon load: 8%
endcap: yes
pH range: 2.5 to 8
temperature limit: 80 °C