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Mutagenic, carcinogenic, and pervasive in the mod-
ern environment, polynuclear aromatic hydrocar-
bons (PAHs) are emitted by many sources of com-
bustion, including heating devices that burn fossil
fuels and internal combustion engines in motor
vehicles. Because they are universal, PAHs are ana-
lyzed in many air, water, and soil matrices.
Environmental samples often contain complex mix-
tures of PAHs that are difficult to resolve, due to
considerable structural similarity among the various
Reproducible Analyses of
Polynuclear Aromatic Hydrocarbons
Using Pinnacle II™ PAH HPLC Columns and
Restek Reference Standards
by Terrence S. Reid, Innovations Team Chemist
✔ Special-purpose HPLC columns resolve 16 PAHs to baseline.
✔ In-house-manufactured silica ensures reproducible column performance.
✔ Three reference standards—choose the concentrations best suited to your need.
Pinnacle II™ PAH 5µm Columns
2.1mm ID 3.2mm ID 4.6mm ID
Length cat.# cat.# cat.# price
100mm — 9219513 9219515
150mm — 9219563 9219565
200mm — 9219523 9219525
250mm 9219572 9219573 9219575
acenaphthene
acenaphthylene
anthracene
benzo(a)anthracene
benzo(a)pyrene
benzo(b)fluoranthene
benzo(k)fluoranthene
benzo(ghi)perylene
chrysene
dibenzo(a,h)anthracene
fluoranthene
fluorene
indeno(1,2,3-cd)pyrene
naphthalene
phenanthrene
pyrene
2,000µg/mL each in methylene chloride, 1mL/ampul
SV Calibration Mix #5 (16 components)
3/90 and 4/89 SOW
Each 5-pk. 10-pk.
31011 31011-510 —
w/data pack
31011-500 31011-520 31111
acenaphthene 1000µg/mL
acenaphthylene 1000
anthracene 1000
benzo(a)anthracene 500
benzo(a)pyrene 500
benzo(b)fluoranthene 500
benzo(k)fluoranthene 500
benzo(ghi)perylene 500
chrysene 500
dibenzo(a,h)anthracene 500
fluoranthene 500
fluorene 1000
indeno(1,2,3-cd)pyrene 500
naphthalene 1000
phenanthrene 500
pyrene 500
In methylene chloride, 1mL/ampul
610 PAH Calibration Mix A
(16 components)
Each 5-pk. 10-pk.
31264 31264-510 —
w/data pack
31264-500 31264-520 31364
acenaphthene 1000µg/mL
acenaphthylene 2000
anthracene 100
benzo(a)anthracene 100
benzo(a)pyrene 100
benzo(b)fluoranthene 200
benzo(k)fluoranthene 100
phenanthrene 100
benzo(ghi)perylene 200
chrysene 100
dibenzo(a,h)anthracene 200
fluoranthene 200
fluorene 200
indeno(1,2,3-cd)pyrene 100
naphthalene 1000
pyrene 100
In methylene chloride:methanol (1:1), 1mL/ampul
610 PAH Calibration Mix B
(16 components)
Each 5-pk. 10-pk.
31455 31455-510 —
w/data pack
31455-500 31455-520 31555
Figure 1
Pinnacle II™ PAH columns provide reproducible, baseline resolution of the 16 PAHs listed in US
EPA Method 610.
Sample:
Inj.: 610 PAH Calibration Mix A (cat.# 31264), 5µL
Sample diluent: methylene chloride:acetonitrile (1:9, v/v)
Column: Catalog #: 9219563
Dimensions: 150 x 3.2mm
Particle size: 5µm
Pore size: 110Å
Conditions:
Mobile phase: A: water; B: acetonitrile
Time: %B
0 40
30 100
40 100
41 40
51 40
Flow: 0.5mL/min
Temp.: 30°C (or ambient)
Det.: UV @ 254nm
LC_0243
Ret. Time (min.) Conc.
Peak List: Lot 1 Lot 2 Lot 3 (µg/mL)
1. naphthalene 11.95 12.17 11.80 100
2. acenaphthylene 13.41 13.65 13.26 100
3. acenaphthene 15.61 15.89 15.45 100
4. fluorene 16.09 16.31 15.92 100
5. phenanthrene 17.41 17.61 17.23 50
6. anthracene 18.80 18.92 18.61 100
7. fluoranthene 20.23 20.39 20.03 50
8. pyrene 21.31 21.46 21.11 50
9. benzo(a)anthracene 24.88 24.87 24.65 50
10. chrysene 25.64 25.54 25.40 50
11. benzo(b)fluoranthene 28.62 28.53 28.35 50
12. benzo(k)fluoranthene 29.97 29.74 29.70 50
13. benzo(a)pyrene 31.26 31.02 30.97 50
14. dibenzo(a,h)anthracene 33.09 32.74 32.81 50
15. benzo(ghi)perylene 34.46 34.24 34.16 50
16. indeno(1,2,3-cd)pyrene 35.19 34.85 34.91 50
analytes. Reversed phase HPLC is the favored
approach for analyses of PAHs because this tech-
nique can discriminate among the closely related
compounds, on the basis of molecular shape. US
Environmental Protection Agency Method 610, for
example, calls for reversed phase HPLC for analyses
of the 16 PAHs listed in Figure 1.
We developed the newest addition to our Pinnacle
II ™ HPLC product line, the Pinnacle II™ PAH col-
umn, specifically for these demanding analyses. The
Pinnacle II ™ PAH stationary phase is a specialized
polymeric C18 bonding that uses unique shape
selectivity to provide baseline resolution of all 16
PAHs in EPA Method 610. Further, we make
Pinnacle II™ PAH columns using silica we manufac-
ture ourselves, at our Bellefonte, PA facility. By mak-
ing our own silica, we have greater control over
both quality and reproducibility. Pinnacle II™ PAH
columns provide the same selectivity as our original
Pinnacle™ PAH columns, but greater reproducibility.
Lot-to-lot reproducibility of performance for
Pinnacle II ™ PAH columns is excellent, as shown in
Figure 1. Each of the three lots of columns repre-
sented has provided baseline resolution of the 16
PAHs listed in Method 610, using a simple, linear
water/acetonitrile mobile phase gradient.
In addition to these special-purpose HPLC columns,
Restek manufactures three analytical reference mixes
for EPA Method 610. The mixes differ in concentra-
tions, but each contains all 16 listed PAHs, packaged
in convenient 1mL ampuls. The sample used to
obtain Figure 1 is 610 PAH Calibration Mix A.
If you are analyzing PAHs, the information present-
ed here shows Restek can provide both the reliable,
cost-effective HPLC columns and the chemical stan-
dards you need.
(Mix A)
lot 1
lot 2
lot 3