ADV01-05_1-298

with data pack

Figure 2

An Ultra Carbamate column provides rapid, excellent results in LC/MS analyses.

Figure 1

An Ultra Carbamate column reduces

analysis time by 70%.

• 10 •

www.restekcorp.com

800-356-1688

The traditional HPLC column for analyses of carba-

mates is a 250 x 4.6mm column containing a 5µm

C18 packing. This column separates 10 target carba-

mates and an internal standard, but the price paid

for the separation is steep—the analysis time is

approximately 35 minutes. Several column manufac-

turers have managed to reduce the analysis time by

packing a 4µm C18 particle in a 150mm column.

This configuration reduces the analysis time to

approximately 25 minutes.

by Greg France, HPLC Marketing Manager, Rebecca Wittrig, Ph.D., Food, Flavor, & Fragrance Innovations Chemist,

and Vernon Bartlett, HPLC Innovations Manager

New Ultra HPLC Column for

Carbamates Analysis

Unique Stationary Phase Separates

10 Carbamates in 10 Minutes

✔

Reduce analysis time by 70% versus a C18 column.

✔

Greatly reduce solvent purchase and disposal costs.

✔

Compatible with LC/MS detection.*

Changing the physical parameters of a column is a

brute force way to improve analyte separation or

reduce analysis time. A better way to improve a

method is to examine the analytes and determine if

an alternative stationary phase or mobile phase

composition would provide a better or faster sepa-

ration. After the optimum stationary phase and

mobile phase are selected, the analysis can be fine-

tuned by adjusting the column length and/or the

particle size.

With a chemistry-based approach in mind, Restek

chemists developed the Ultra Carbamate column

specifically for carbamates analysis. The new col-

umn has a unique stationary phase that, when

bonded to 3µm silica, creates a packing that sepa-

rates 10 target carbamates in under 10 minutes

(Figure 1). The new stationary phase is compatible

with LC/MS detection (Figure 2). The mobile phas-

es used to obtain these separations are binary gra-

dients consisting of water, methanol, and acetoni-

trile, without (Figure 1) or with ammonium for-

mate (Figure 2).

An Ultra Carbamate column can process as many as

3 to 4 samples per hour, versus less than 2 samples

per hour on a traditional C18 column. In addition

to increased sample throughput, faster analyses sig-

nificantly reduce solvent usage—and the costs of

disposing of the solvent waste after the analysis. If

you have been analyzing carbamates with a general-

purpose C18 column, we think you will be highly

impressed with the performance of an Ultra

Carbamate column—and the increased sample

throughput and solvent savings will quickly pay for

your new column.

*Also compatible with post-column derivitization and fluores-

cence detection, if total system dead volume is less than 650µL,

inclusive of post-column reactors.

Column:

Ultra Carbamate

Catalog #:

9177355

Dimensions:

50 x 4.6mm

Particle size:

3µm

Pore size:

100Å

Conditions:

Inj.:

5µL

Conc.:

50µg/mL

Solvent:

methanol

Restek standards:

Catalog# 32274 (IS) and 32273 mixed 50:50

Mobile Phase:

A: 90:10 water:methanol

B: 90:10 methanol:acetonitrile

Time (min):

Flow:

1.5mL/min

Temp.:

27°C

Det.:

UV @ 220nm

LC_0225

1. aldicarb sulfone

2. aldicarb sulfoxide

3. oxamyl

4. methomyl

5. 3-hydroxycarbofuran

6. aldicarb

7. propoxur

8. carbofuran

9. carbaryl

10. methiocarb

11. 4-bromo-3,5-dimethyl-

phenyl-N-methyl-

carbamate (BDMC) (IS)

70%

faster!

Column:

Ultra Carbamate

Catalog #:

9177315

Dimensions:

100 x 4.6mm

Particle size:

3µm

Pore size:

100Å

Inj.:

10µL

Sample:

531.1 Carbamate

Pesticide Calibration Mix

(cat.# 32273)

Conc.:

100µg/mL each

Solvent:

methanol

Mobile Phase:

A: 90:10 water:methanol + 10mM

ammonium formate

B: 10:90 acetonitrile:methanol + 10mM

ammonium formate

Gradient:

90:10 A:B to 10:90 A:B

from 0-15 min.

Flow:

1mL/min. (0.75mL/min. to

UV, 0.25mL/min. to MS)

Temp.:

ambient

MS Conditions:

Det.:

Micromass ZMD

Mode:

ESI+

Capillary V:

3.50

Extractor:

4.0

Ion Energy:

0.4

Multiplier:

650

Source Temp.:

100ºC

Desolv. Temp.:

250ºC

Gas Flow:

490 L/hr.

Compound

Ion

Cone V

1. aldicarb sulfone

223.3

25V

2. aldicarb sulfoxide

207.3

18V

3. oxamyl

237.2*

10V

4. methomyl

163.2

15V

5. 3-hydroxycarbofuran 238.3

15V

6. aldicarb

191.2

7. propoxur

210.2

18V

8. carbofuran

222.3

22V

9. carbaryl

202.2

18V

10. methiocarb

226.3

19V

*Ammonium adduct;

others [M+H]+ ions.

Ordering Information

| 3µm Ultra Carbamate

HPLC Columns

Description

Length 4.6mm ID

Ultra Carbamate

50mm 9177355

Ultra Carbamate

100mm 9177315

aldicarb

aldicarb sulfone

aldicarb sulfoxide

carbaryl

carbofuran

3-hydroxycarbofuran

methiocarb

methomyl

oxamyl

propoxur (baygon)

100µg/mL each in methanol, 1mL/ampul

531.1 Carbamate Pesticide Calibration

Mixture

(10 components)

Each

5-pk.

10-pk.

32273

32273-510

—

32273-500

32273-520

32373

4-bromo-3,5-dimethylphenyl-N-methylcarbamate (BDMC)

100µg/mL in methanol, 1mL/ampul

Internal Standard

Each

5-pk.

10-pk.

32274

32274-510

—

32274-500

32274-520

32374