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www.restekcorp.com

800-356-1688

Restek HPLC Column Kits for Faster,

Easier Method Development

by Rebecca Wittrig, Ph.D., HPLC Product Marketing Manager

✔

Multiple stationary phases, for quick optimization of selectivity.

✔

Specific kits for MS or UV detection—both include columns specifically for basic analytes.

✔

Fast LC kits use economical cartridge design.

When developing a new HPLC assay, method devel-

opment chemists often start with a C18 or C8 station-

ary phase, because these phases have proven useful

for analyzing a wide range of organic compounds.

Many analysts have learned, however, that a C18 or

C8 stationary phase is not the best choice for every

separation. A cyano-, pentafluorophenyl, or amino-

containing stationary phase, a phase with embedded

polar groups, or a phase designed for compatibility

with highly aqueous mobile phases might provide

superior resolution of target compounds (Figure 1).

To help analysts efficiently select the optimum sta-

tionary phase, we have assembled kits of columns

for use in HPLC method development. The four

columns in each kit incorporate a range of station-

ary phase types, are configured for rapid analyses,

and are optimized for the detector type: 50 x 4.6mm

ID columns containing 5µm packings for use with

UV detection, 30 x 2.1mm ID columns containing

3µm packings for LC/MS. The Fast LC method devel-

opment kits include four 30 x 2.1mm ID or four 30

x 4.0mm ID cartridges containing 3µm packings,

and a cartridge holder.

Description

qty.

cat.# price

LC/MS (1 Allure

™

C18 (USP L1), 1 Allure

™

PFP Propyl,

1 Allure

™

Basix (USP L10), 1 Ultra Aqueous C18 (USP L1))

four 30 x 2.1mm, 3µm columns 25136

LC/UV (1 Pinnacle

™

DB C18 (USP L1), 1 Allure

™

PFP Propyl,

1 Ultra Aqueous C18 (USP L1), 1 Ultra IBD)

four 50 x 4.6mm, 5µm columns 25137

Fast LC Method Development Kit

Each kit includes four Fast LC Cartridges (Ultra C18 (USP L1), Ultra Aqueous C18 (USP L1), Ultra Cyano (USP

L10), Ultra PFP (USP L43)) and a Fast LC cartridge holder.

Description

qty.

cat.# price

Fast LC Development Kit

four 30 x 2.1mm columns

25296

Fast LC Development Kit

four 30 x 4.0mm columns

25297

High Performance

Silica Products

(lit. cat.# 59901)

Tight production

specifications ensure

highly consistent lot-to-lot

performance from Restek's

proprietary silica materi-

als. This 6-page publication

characterizes Pinnacle II

™

and Pinnacle

™

DB stock

bulk products—bare silica and silica with popular

bonded phases—and illustrates typical results

achieved by using them. Custom materials can be

designed from a broad range of particle sizes, pore

sizes, and phases.

HPLC Method

Development Course

Many analysts anticipate developing a new HPLC

method with apprehension. To help de-mystify this

process, Restek, in cooperation with ChromVision,

offers a dynamic, thorough two-day course on the sub-

ject, presented in a logical and systematic manner by

an expert on the effects of adsorbent chemistry and

structure on HPLC retention. It provides the knowledge

and tools necessary for understanding why a particular

stationary phase would be chosen for separating vari-

ous analytes, and how to improve selectivity by choos-

ing the proper eluent. Eight lectures cover all aspects

of method development and are complemented and

reinforced by four workshops. The course is especially

useful to analysts working with pharmaceutical or bio-

logical/biochemical analytes. For more information

about this and other Restek seminars, visit our semi-

nars web page:

www.restekcorp.com/seminar

Bulk Silica Available

Figure 1

Better chromatography for basic molecules, using an Ultra IBD column.

The base-deactivated column has a unique blend of hydrophobic and polar character for better

resolution of closely related compounds.

Peak List:

1. uracil

2. famotidine

3. ranitidine

Ultra IBD Phase

• High Selectivity

• Marked Resolution

Columns:

Dimensions:

150 x 4.6mm

Particle Size:

5µm

Pore Size:

100Å

Conditions:

Mobile Phase: 20mM K

pH 7:acetonitrile (70:30,v/v)

Flow Rate:

1.0mL/min

Det.:

UV @ 234nm

2, 3

Conventional C18 Phase

• Little Retention

• No Resolution

HPLC and LC/MS Column Kits

12 min.

14 min.

If the columns in the kits listed below don’t appear

to meet your needs, please contact our Technical

Service group or your Restek distributor for infor-

mation about custom kits.