Theuniform spherical shapeof Restek
silicaallowsoptimal packing, for better
reproducibilityand lower backpressure,
resulting inextended column life.
HPLCColumnSelectionGuide
INNOVATIVE
PRODUCTS
RestekCorporationmanufactures a variety of columns for reversedphase or normal
phase applications. TheAllure™andUltra columnproduct lines contain 19 different
phases, andwe continue todevelop additional uniqueHPLCproducts. Pinnacle
II™ andPinnacle™DB columns are basedon silicawemanufacture inour
own laboratories, so you canbe assuredof their quality, from rawmaterial
synthesis throughpacking and testing.
We pack bondedphases into awide range of columndimensions,
including standard analytical columns in lengths of 30 to 250mm,
internal diameters of 1.0 to 4.6mm, andparticle sizes of 3 or 5µm.
Preparative and semi-preparative columns are available, aswell.
These hundreds of column choices canmake column selection
overwhelming. The chart below, and the informationon the back
of the chart, are general guidelines tohelp simplify the column
selectionprocess.
20
YEARS
TurningVisions intoReality
™
800-356-1688 • 814-353-1300
Restek
HPLCColumn
End
Cap?
Pore
Size
(Å)
Carbon
Load (%) Applications
ChromatographicProperties
Similar Phases
USP
Code
Pinnacle
™
DB
C18
Y
140
11
HydrophobicC18phase suitable for awide rangeof
compounds, neutral throughweaklybasic.
Highlybase-deactivated spherical silicamanufactured
byRestekCorp.MonomericC18bonding.
Hypersil
®
BDSC18
L1
Pinnacle
™
DB
C8
Y
140
6
Applications similar toPinnacle
™
DBC18, butwith less
hydrophobic retention. Less retention canbeuseful for
shorteninganalysis time, if resolution is adequate.
Highlybase-deactivated spherical silicamanufacturedbyRestekCorp.
MonomericC8bonding. Similar toPinnacle
™
DBC18, but the shorter
alkyl chainprovides lesshydrophobic retention.
Hypersil
®
BDSC8
L7
Pinnacle
™
DB
Cyano
Y
140
4
Suitable for awide rangeof compounds, fromacidic
throughweaklybasic. Alsouseful for confirmationof
analysesonC18or C8. Canbeused innormal phaseor
reversedphasemodeof separation.
Cyanobondingonhighlybase-deactivated spherical silica
manufacturedbyRestekCorp.
Hypersil
®
BDSCyano
L10
Pinnacle
™
DB
Silica
NA 140
NA Normal phase separations.
Highlybase-deactivated spherical silicamanufactured
byRestekCorp.
Hypersil
®
BDS
L3
Pinnacle
II
™
C18
Y
110
13
Superior general purposeC18 for neutral andacidic
analytes.
Intermediate carbon loadand surfacearea, suitable for awide range
of neutral toacidic compounds. SilicamanufacturedbyRestekCorp.
Hypersil
®
ODS;
Spherisorb
®
ODS
L1
Pinnacle
II
™
PAH
Y
110
P
Maximum resolutionof polynuclear aromatic
hydrocarbons.
Proprietary stationaryphase; resolves 16PAHs inUSEPAMethod610.
SilicamanufacturedbyRestekCorp.
Unique
Pinnacle
II
™
C8
Y
110
7
Superior general purposeC8 for neutral andacidic
analytes.
Provides shorter retention times for hydrophobic compounds
thanC18. SilicamanufacturedbyRestekCorp.
Hypersil
®
C8
L7
Pinnacle
II
™
Cyano
Y
110
4
Superior general purpose cyano forweaklybasic
analytes. Canbeused innormal phaseor reversed
phasemodeof separation.
More rugged thanbare silica for normal phaseanalyses.
SilicamanufacturedbyRestekCorp.
Hypersil
®
Cyano;
Spherisorb
®
Cyano
L10
Pinnacle
II
™
Phenyl
Y
110
6
Superior general purposephenyl for neutral toweakly
basic analytes.
Offersunique selectivityversus traditional alkyl chainphases,
especially for aromatic compounds. Silicamanufactured
byRestekCorp.
Hypersil
®
Phenyl;
Spherisorb
®
Phenyl
L11
Pinnacle
II
™
Amino
N 110
2
Excellent general purposeaminophase.
Excellent choice for carbohydrateanalysis.
SilicamanufacturedbyRestekCorp.
Hypersil
®
Amino;
Spherisorb
®
Amino
L8
Pinnacle
II
™
Silica
NA 110
NA
Superior valuephase for normal phase separation
of polar analytes.
Lower retention thanUltraC18. SilicamanufacturedbyRestekCorp.
Hypersil
®
Silica
L3
Allure
™
C18
Y
60
27
Ideal forMSand light-scatteringdetectionof neutral
to slightlypolar non-ionic solutes. Excellent for basic
compounds, explosives, or steroids.
Most retentivephase for hydrophobic and slightlypolar analytes.
Mobilephase containinghigher percentageof organicmodifier
contributes tohigher sensitivity inESI-basedLC/MS. Extensive
deactivation.
Ultracarb
®
C18;
Betamax
®
Neutral
L1
Allure
™
AqueousC18
N 60
P
Ideal for analyses that require>90%water in the
mobilephase. Excellent for highlywater solubleor
poorlyorganic soluble compounds,water-soluble
vitamins, organicacids.More retention thanUltra
Aqueous columns.
Highly retentiveand selective for reversedphase separationsof
polar analytes. Highlybasedeactivated. Compatiblewithhighly
aqueous (up to100%)mobilephases.
L1
Allure
™
Basix
Y
60
12
Ideal for LC/MSof basic solutes. Excellent for basic
pharmaceuticalsor other amine-containing compounds.
Highly retentivephase for analytes containingamino functionality.
Betamax
®
Base;
Maxsil
®
CN
L10
Allure
™
PFP
Propyl
Y
60
17
Excellent for beta-blockers, halogenated compounds,
nucleosides, nucleotides, pyridines, pyrimidines, or
tricyclic antidepressants. Ideal forMS, ELSD, orNPD
detection.
Apentafluorophenyl phasewithapropyl spacer. Highly retentive
for basic analytes.
DiscoveryHSF5
Allure
™
Organic
Acids
N 60
P
Excellent resolutionof challengingorganic acids.
Single30cm columnperforms equally to twoC18 columns in series.
(AOACMethod986.13)
Unique
Allure
™
Silica
NA 60
NA
Highly retentivephase for polar analytesbynormal
phase separation.
Highpurity, veryhigh surfacearea.
Maxsil
®
Si
L3
UltraC18
Y
100
20
Ideal for anilines, barbiturates, carbonyls, fat-soluble
vitamins, fattyacids, glycerides, phthalates, PTHamino
acids, steroids, other acids.
Very retentive, high-puritypackingmaterial exhibits excellent
peak shape for awide rangeof compounds. Excellent first choice
general purpose reversedphase column.
DiscoveryC18;
SymmetryC18;
Luna
®
C18; Zorbax
®
C18;
Kromasil
®
C18;
Lichrospher
®
RP-18;
Inertsil
®
ODS-2; Develosil
®
C18
L1
UltraAqueous
C18
N 100
15
Ideal for analyses that require>90%water in the
mobilephase. Excellent for highlywater solubleor
poorlyorganic soluble compounds,water-soluble
vitamins, organic acids.
Highly retentiveand selective for reversedphase separationsof
polar analytes. Extensivelybasedeactivated. Compatiblewith
highlyaqueous (up to100%)mobilephases.
ODS-AQ
Aquasil C18
Zorbax
®
SB-AQ
L1
Ultra IBD
N 100
12
Apolar groupassists indeactivating surface silanols
and contributes tounique selectivities for acids, bases,
zwitterions, andpolar compounds.
Intrinsicallybase-deactivated (IBD) phase,withapolar group
embedded in thealkyl bondedphase. Providesunique selectivity
while reducingor eliminating theneed formobilephaseadditives.
SymmetryShield;
DiscoveryABZ&ABZ+;
Prism
®
; HyPurityAdvance
UltraC8
Y
100
12
Excellent peak shape for awide rangeof compounds.
Selectivityandpeak shape similar toUltraC18, but less
hydrophobic retention.
Very retentive, high-purity, base-deactivated reversedphase
packing.
Luna
®
C8; SymmetryC8;
Kromasil
®
C8
L7
UltraC4
Y
100
9 Ideal for peptides, small proteins, hormones.
Exceptionally stableC4packing,withhighbonding coverageand
silanol base-deactivation. Shorter retention thanC18or C8.
Supelcosil Butyl (C4);
Delta-Pak
®
C4
L26
UltraC1
NA 100
5
Alternative selectivity toUltraC18or C8, especially for
polar analytes. Shortest chainalkyl phaseavailable for
reversedphase separations.
Exceptionally stableC1packing resistshydrolysis, evenunder acidic
mobilephase conditions. Least retentive reversedphasehydrocarbon
packing.
Spherisorb
®
C1
L13
UltraCyano
Y
100
8
Excellent for basicpharmaceuticals, steroids (normal or
reversedphase conditions), or other basic compounds.
High-purity cyanophasewith reduced silanol activity. Oftenabetter
choice thanC18 for basicpharmaceuticals. Cyano is themost stable
bondedphase for normal phase separations.
Platinum
®
CN;
Develosil
®
Cyano;
Luna
®
CN
L10
UltraPhenyl
Y
100
10
Ideal for fattyacids, polynuclear aromatichydrocarbons,
purines andpyrimidines, polar aromatics.
High-purity, highly retentive, base-deactivatedphasewithalternate
selectivity tohydrocarbonphases, especially for aromatic analytes.
Platinum
®
Phenyl;
Supelcosil Phenyl
L11
UltraAmino
N 100
2
Superior general purposeaminophase.
Ideal for carbohydrates.
Recommended for normal phaseanalysesofmono- anddisaccharides
andother similar compounds. Canalso serveas aweakanion
exchanger,withaqueousbuffers.
Platinum
®
Amino;
Develosil
®
NH
2
L8
UltraPFP
Y
100
7
Ideal for taxol andprecursors, halogenated compounds,
amines, esters, or ketones.
Apentafluorophenyl phase. Unique selectivityby interactionwith
functional groupsof organohalogensor other basic analytes.
Fluophase
®
PFP;
Fluosep-RP
®
Phenyl
L43
UltraSilica
NA 100
NA Ideal for normal phaseapplications.
Highpurity, high surfacearea.
Unique
L3
Ultra
Carbamate
P
100
P Rapidanalysisof carbamates.
Proprietary stationaryphase canprocessup to twiceasmany
samplesper hour, compared toa conventional C18phase.
Unique
UltraQuat
P
100
P
Proprietaryphase for paraquat, diquat, andother
quaternaryamines.
Highpurity silica. Requires a reagent solution.
Unique
—
Viva
™
WidePore
C18
Y
300
6
Proteins andother highermolecularweight
compounds.
SilicamanufacturedbyRestekCorp.
Symmetry
®
300C18;
Jupiter
®
300C18;
BioBasic
®
C18;
Zorbax
®
300SB-C18
Viva
™
WidePore
Silica
NA 300
NA
Highermolecularweight compoundsbynormal
phasemode.
SilicamanufacturedbyRestekCorp.
Symmetry
®
300 silica; Jupiter
®
300 silica; Zorbax
®
300 silica
P= proprietary
NA= not applicable
All columns: recommended pH range is 2.5–7.5; temp. limit 80°C.
Column lifetimes can be shortened by operating at pH and/or temperature extremes.
Call RestekNow for theRight Column for
YourToughAnalysis 1-800-356-1688
