While“1s and 5s”are oftenused initially inGC/MSmethoddevelopment because of their thermal stability, their nonpolar charac-
ter results inpoor retention for polar compounds and costs additional development time. In contrast,midpolarityRxi®-624SilMS
columns provide improved retention and selectivity for polar compounds and are alsomore compatible with polar injection sol-
vents.Highly volatile, polar alkyl halide genotoxic impurities, for example, are difficult to retain on 1s and 5s, but the Rxi®-624Sil
MS columnprovides higher retention capacity,makingGC/MS analysis easier to control and allowing fastermethoddevelopment
(Figure 1).
Figure1
Polar compounds, suchasalkyl halides, arehighly retainedonmidpolarityRxi®-624SilMScolumns,making
methoddevelopment faster andeasier thanonanonpolar 1or 5 typecolumn.
Enhanced retention forhard-to-retainpolar
analytesmakesmethoddevelopmenteasier!
GC_PH1166
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for
detailed comparisons and to learn
howexceptional Rxi® inertness,
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theRightColumnFirst
Peaks
RT (min.)
1. 2-Chloropropane
2.10
2. Bromoethane
2.36
3. 1-Chloropropane
2.72
4. 2-Bromopropane
3.393
5. Butyl chloride
4.627
6. 1-Bromobutane
5.973
Column
Rxi
®
-624Sil MS, 20m, 0.18mm ID, 1.00µm (cat.# 13865)
Sample
Diluent:
DMSO
Conc.:
1µg/mL each compound
Injection
Inj. Vol.:
1µL splitless (hold 0.5min.)
Liner:
3.5mm SingleGooseneck Liner withwool placed 3cm
from top (middle) (cat.# 22286)
Inj.Temp.:
220 °C
Purge Flow:
3mL/min.
Oven
OvenTemp:
40 °C (hold 3min.) to 200 °C at 20 °C/min.
Carrier Gas
He, constant flow
Linear Velocity:
40 cm/sec.
Detector
MS
Mode:
Scan
Transfer LineTemp.: 280 °C
AnalyzerType:
Quadrupole
SourceTemp.:
280 °C
Solvent DelayTime: 0.5min.
IonizationMode:
EI
Scan Range:
30-300 amu
Scan Rate:
5 scans/sec.
Instrument
Shimadzu 2010GC&QP2010+MS
Notes:
EIC of 42, 43, 57, 108m/z
BalancedRetentionSimplifiesMethodDevelopmentForPolar Impurities
Theversatilityof anRxi®-624SilMS
columnmakes itaperfect fit for
QualityByDesign.
3
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