Restek-2015

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LC COLUMNS
Columns for Bioseparations
Viva HPLC Columns: 3 or 5 µm particles; 300 Å pore size
• Excellent for separating peptides or proteins.
• Rugged, spherical particles with 300 Å pore size.
• High proportion of pore/surface area available to large molecules.
Viva columns are based on a wide pore material we designed for optimal large-molecule separations. In developing Viva silica, we
found that although many commercial wide-pore silicas meet the standard 300 Å mean pore size, most have very broad distribu-
tions about this mean, with a significant portion of their pore volume falling below 150 Å. This means a large portion of the surface
area is unavailable to larger molecules. Viva columns have a narrow distribution around the mean pore size, permitting a larger
portion of the silica surface to play a role in the separation.
Restek-
manufactured
silica
Restek-
manufactured
silica
Column Characteristics:
particle size: 3 µm or 5 µm,
spherical
pore size: 300 Å
carbon load: 9%
end-cap: yes
pH range: 2.5 to 8
temperature limit: 80 °C
USP phase code: L1
phase category: C18,
octadecylsilane
ligand type: monomeric C18
Si CH3 CH3
O
C18
Column Characteristics:
particle size: 5 µm, spherical
pore size: 300 Å
carbon load: 5%
end-cap: yes
pH range: 2.5 to 8
temperature limit: 80 °C
USP phase code: L7
phase category: C8, octylsilane
ligand type: monomeric C8
Si CH3 CH3
O
C8
Restek-
manufactured
silica
Chromatographic Properties
The general-purpose Restek® C18 is a conventional monomeric octadecylsilane column suitable
for analyses of a wide range of compounds from acidic through slightly basic.
Viva C18 Columns (USP L1)
Chromatographic Properties
Our C8 is a conventional monomeric octylsilane column offering a shorter alkyl chain to pro-
vide less hydrophobic retention and improved basic peak shape over a traditional C18 phase.
Like our C18, this general-purpose Restek® C8 is suitable for a wide range of compounds from
acidic through slightly basic.
Viva C8 Columns (USP L7)
1.0 mm ID 2.1 mm ID 3.0 mm ID 4.6 mm ID
Length cat.# price cat.# price cat.# price cat.# price
3 µm Columns
30 mm 9514331 9514332 951433E 9514335
50 mm 9514351 9514352 951435E 9514355
100 mm 9514311 9514312 951431E 9514315
150 mm 9514361 9514362 951436E 9514365
5 µm Columns
30 mm 9514531 9514532 951453E 9514535
50 mm 9514551 9514552 951455E 9514555
100 mm 9514511 9514512 951451E 9514515
150 mm 9514561 9514562 951456E 9514565
200 mm 9514521 9514522 951452E 9514525
250 mm 9514571 9514572 951457E 9514575
1.0 mm ID 2.1 mm ID 3.0 mm ID 4.6 mm ID
Length cat.# price cat.# price cat.# price cat.# price
5 µm Columns
30 mm 9513531 9513532 951353E 9513535
50 mm 9513551 9513552 951355E 9513555
100 mm 9513511 9513512 951351E 9513515
150 mm 9513561 9513562 951356E 9513565
200 mm 9513521 9513522 951352E 9513525
250 mm 9513571 9513572 951357E 9513575