172 / 628
170
www.restek.comLC COLUMNS
Columns for HPLC
Steroid Panel Analysis on the Ultra Biphenyl
➲
For conditions, visit
www.restek.comand
search for LC_CF0537.
Column Characteristics:
particle size:
3 µm or 5 µm,
spherical
pore size:
100 Å
carbon load:
15%
end-cap:
yes
pH range:
2.5 to 8
temperature limit: 80 °C
USP phase:
L11
phase category:
phenyl
ligand type:
unique Biphenyl
Biphenyl
Si
CH
3
CH
3
O
Biphenyl
D
i
s
p
e
r
s
i
o
n
H
-
B
o
n
d
i
n
g
P
o
l
a
r
i
z
a
b
i
l
i
t
y
C
a
t
i
o
n
E
x
c
h
a
n
g
e
USLC® Column Interaction Profile
(See
page 161for more information.)
Biphenyl
Si
CH
3
CH
3
O
1
2
3
15
16
4
5
6
7
8
9
10
11
12
13
4.3 4.4 4.5 4.6 4.7 4.8 4.9 5.0 5.1 5.2 5.3 5.4 5.5 5.6
0.5
0.0
1.0
1.5
2.0
2.5
3.0
3.5
4.0 4.5
5.0
5.5
6.0
6.5
7.0
7.5
8.0
8.5
9.0
9.5
Time (min)
14
LC_CF0537
Chromatographic Properties
Since 2005, the Restek® Biphenyl has offered a greater degree of dispersion than conventional
phenyls and a greater degree of polarizability than phenyl hexyls, creating higher selectivity and
a greater range of usability. Because of these heightened interactions, this column shows sub-
stantial increases in retention—especially for dipolar, unsaturated, or conjugated solutes—and
enhanced orthogonal selectivity when using methanol mobile phases. It is ideal for increasing
sensitivity and selectivity in LC-MS analyses.
Ultra Biphenyl Columns
(USP L11)
2.1 mm ID
3.0 mm ID
4.6 mm ID
Length
cat.#
price
cat.#
price
cat.#
price
3 µm Columns
30 mm
9109332
910933E
9109335
50 mm
9109352
910935E
9109355
100 mm
9109312
910931E
9109315
150 mm
9109362
910936E
9109365
5 µm Columns
30 mm
9109532
910953E
9109535
50 mm
9109552
910955E
9109555
100 mm
9109512
910951E
9109515
150 mm
9109562
910956E
9109565
200 mm
9109522
910952E
9109525
250 mm
9109572
910957E
9109575
Peaks
t
R
(min)
1. Cortisol
2.3
2. Estradiol
2.6
3. 11-Deoxycortisol
4.0
4. DHEA
4.3
5. Boldenone
4.4
6. Corticosterone
4.4
7. Stanozolol
4.4
8. Epitestosterone
4.5
9. 17-OH-progesterone 4.8
10. Testosterone
5.0
11. Trenbolone
5.1
12. Methyltestosterone
5.1
13. Nandrolone
5.3
14. 25-Hydroxyvitamin D3 6.1
15. Androstenedione
6.2
16. Progesterone
7.5