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REFERENCESTANDARDS|
FOODS, FLAVORS&FRAGRANCESMATERIALS
Derivatization Reagents
Compound
CAS# cat.#
price
MBTFA (N-methyl-bis-trifluoroacetamide)
10-pk. (10x1g)
685-27-8
35616
25g vial
685-27-8
35617
TFAA (trifluoroacetic acid anhydride)
10-pk. (10x1g)
407-25-0
35618
25g vial
407-25-0
35619
PFAA (pentafluoropropionic acid anhydride)
10-pk. (10x1g)
356-42-3
35620
25g vial
356-42-3
35621
HFAA (heptafluorobutyric acid anhydride)
10-pk. (10x1g)
336-59-4
35622
25g vial
336-59-4
35623
PFPOH (pentafluoropropanol)
10-pk. (10x1g)
422-05-9
35624
25g vial
422-05-9
35625
AcylationDerivatizationReagents
Acylation reagents offer the same types of advantages available
from silylation reagents: creating less polar,more volatilederiva-
tives. In comparison to silylating reagents, the acylating reagents
can more readily target highly polar multi-functional com-
pounds, such as carbohydrates and amino acids. In addition,
acylating reagents offer the distinct advantage of introducing
electron-capturing groups, thus enhancing detectability during
analysis.
• Most commonlyused for electron capture detection.
• Reactwith alcohols, amines andphenols.
• Frequentlyused for drugs of abuse confirmation.
Compound
CAS# cat.#
price
TMPAH
10-pk. (10x1g)
1899-02-1
35614
25g vial
1899-02-1
35615
AlkylationDerivatizationReagents
Alkylation reagents reducemolecularpolarityby replacingactive
hydrogens, such as carboxylic acids and phenols. Alkylation
reagents canbeusedalone to form esters andamidesor they can
be used in conjunction with acylation or silylation reagents. A
two-step approach is commonly used in the derivatization of
amino acids, where multiple functional groups of these com-
poundsmaynecessitate protectionduringderivatization.
Esterification is the reaction of an acid with an alcohol in the
presenceof acatalyst. It is themostpopularmethodof alkylation
due to theavailabilityof reagents and easeof use.Alkyl esters are
stable, and can be formed quickly and quantitatively. Retention
of the derivative canbe variedby altering the lengthof the sub-
stituted alkyl group. In addition to the formation of simple
esters, alkylation reagents can be used in extraction procedures
where biologicalmatrices are present.
• Adds alkyl groups to functional hydrogens (H).
• Decreases polarityon compounds containing acidic
hydrogens, i.e., phenols, carboxylic acids.
• Forms an ester.
• Replaces active hydrogen, reducingpolarity andmaking the
compoundsmore volatile.
• Increases stabilityof derivatives.
Compound
CAS# cat.#
price
MSTFA (N-methyl-N-trimethylsilytrifluoroacetamide)
10-pk. (10x1g)
24589-78-4
35600
25g vial
24589-78-4
35601
MSTFAw/1%TMCS (N-methyl-N-trimethylsilytrifluoroacetamidew/1%
trimethylchlorosilane)
10-pk. (10x1g)
24589-78-4
35602
25g vial
24589-78-4
35603
BSTFA (N,O-bis[trimethylsilyl]trifluoroacetamide)
10-pk. (10x1g)
25561-30-2
35604
25g vial
25561-30-2
35605
BSTFAw/1%TMCS (N,O-bis[trimethylsilyltrifluoroacetamide]
w/1% trimethylchlorosilane)
10-pk. (10x1g)
25561-30-2
35606
25g vial
25561-30-2
35607
MTBSTFAw/1%TBDMCS (N-methyl-N[
tert
-butyldimethylsilyl trifluoroacetamide]
w/1%
tert
-butyldimethylchlorosilane)
10-pk. (10x1g)
77377-52-7
35608
25g vial
77377-52-7
35610
TMCS (trimethylchlorosilane)
10-pk. (10x1g)
75-77-4
35611
25g vial
75-77-4
35612
SilylationDerivatizationReagents
Silylation is the most widely used derivatization procedure for
sample analysis by GC. In silylation, an active hydrogen is
replaced by an alkylsilyl group such as trimethylsilyl (TMS) or
tert
-butyldimethylsilyl (
tert
-BDMS). Silyl derivatives are more
volatile, less polar, and more thermally stable. As a result, GC
separation is improved anddetection is enhanced.
BothTMS and
tert
-BDMS reagents are suitable for awide vari-
ety of compounds and can be used for many GC applications.
Note that silylation reagents aregenerallymoisture sensitive and
must be sealed toprevent deactivation.
Derivatization
Reagents
• Reagentsavailable for acylation,
alkylation, and silylation.
• Packaged in10x1gvialsor 25gvials.
• Highpurity for accurate results.
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Mar 2011
