POPULAR CONFIGURATION GCs
Gas-less
TM
Educational CCD
General Operating Procedure
1. ConnectyourGC toyourWindowsPCwithPeakSimple installed. Plug inyourGCand turn itspoweron.
2. Set theColumnOven temperature to130
o
C inPeakSimpleasfollows:
InitialTemp
Hold
Ramp
FinalTemp
130.00
10.00
0.00
130.00
Inan isothermaloperation like this, theHoldperioddetermines the lengthof theanalyticalrun.
NOTE:
TheHaysepDpackedcolumn isstandardfor thisGCsystembecauseof itsseparation
qualitiesanddurability. Toavoidpossibledamage to thepacking,donotprogramyourColumnOven
toheat above150
o
C.
3. Let thesystemstabilize forat least10minutes, allowing theCCDdetector toadjust to the increase in
temperature.
HayesepDpackedcolumnsareusefulforanalyzinggases
and lowmolecularweightcompoundssuchasalcohols,
aldehydes, andketones. For heaviermolecularweight
liquids,usea30mor60mMXT-1capillarycolumn.
1mHayesepDpackedcolumn
30mMXT-1 .53mm capillarycolumn
Column Notes
AutoZero
button
4. Clickon theZerobutton to the left of the chromatogramwindow in
PeakSimple tozeroout theDataSystemsignal. Otherwise, thesignal
startsout at1000millivolts. Press theRUNbuttononyourGCor the
spacebaronyourcomputerkeyboard tobegin therun.
5. Injectsample into theOn-ColumnInjector. A1µL1000ppmmethanol/acetonesample is thefactory test
standardforthisconfiguration.
