• ReversedPhaseHPLC columns aremanufactured
from awide range of silicas that vary inmany
chemical andphysical properties. Differences in
the properties of the underlying silicamayhave a
major influence on the resulting chromatographic
properties of the finished column. Important
physical properties include particle size
distribution, pore size distribution, pore volume,
and the surface area. An important chemical
property is the concentration ofmetals.
