Introduction
There are several variables to consider inHPLC
column selection, but themost critical one is the
stationary phase. Stationary phases canbe broadly
classified by the primary ligand such as octadecyl
(C18), octyl (C8), cyano, and phenyl. However there
aremany other variables that can play a critical role
in selectivity such as ligand density anddegree of
polymeric character.
