restekapp07 - page 91

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UltraC8, 3µmColumns
1.0mm ID 2.1mm ID 3.2mm ID 4.6mm ID
Length: cat.# cat.# cat.# cat.#
30mm 9103331 9103332 9103333 9103335
50mm 9103351 9103352 9103353 9103355
100mm 9103311 9103312 9103313 9103315
UltraC8, 3µmColumns withTrident ™ Inlet Fitting
2.1mm ID 3.2mm ID 4.6mm ID
Length: cat.# cat.# cat.#
30mm — 9103332-700 9103333-700 9103335-700
50mm — 9103352-700 9103353-700 9103355-700
100mm — 9103312-700 9103313-700 9103315-700
UltraC8, 5µmColumns
1.0mm ID 2.1mm ID 3.2mm ID 4.6mm ID
Length: cat.# cat.# cat.# cat.#
30mm 9103531 9103532 9103533 9103535
50mm 9103551 9103552 9103553 9103555
100mm 9103511 9103512 9103513 9103515
150mm 9103561 9103562 9103563 9103565
200mm 9103521 9103522 9103523 9103525
250mm 9103571 9103572 9103573 9103575
UltraC8, 5µmColumns withTrident ™ Inlet Fitting
2.1mm ID 3.2mm ID 4.6mm ID
Length: cat.# cat.# cat.#
30mm — 9103532-700 9103533-700 9103535-700
50mm — 9103552-700 9103553-700 9103555-700
100mm — 9103512-700 9103513-700 9103515-700
150mm — 9103562-700 9103563-700 9103565-700
200mm — 9103522-700 9103523-700 9103525-700
250mm — 9103572-700 9103573-700 9103575-700
©Copyright 2000, RestekCorporation
Summary
The analysis of vanillin and ethyl vanillin in vanilla flavors can
be performed efficiently by liquid chromatography using anUltra
C8 column andUVdetection at 254nm. The separation can be
carried out using either an isocratic or a gradient elution program;
however, in the gradient mode the run time is significantly
reduced and less solvent is needed. In addition, much less peak
broadening is observedwith the gradient program, resulting in
higher sensitivity for the later-eluting compounds.
References
1.AOACOfficialMethods ofAnalysis (2000), 17th edition,
method990.25.
2.Brandt, Laura. “TheCreation andUseofVanilla,”Food
ProductDesign (1996), editorial archives.