environmental
Applications
note
RestekCorporation • (800) 356-1688 • (814)353-1300 •
#59547
environmental
GCAnalysis of USEPAMethod 8081AChlorinatedPesticides
UsingRtx
®
-CLPesticides&Rtx
®
-CLPesticides2Columns
Figure1
Excellent Separation of USEPAMethod 8081AChlorinatedPesticidesUsing
Restek’sRtx
®
-CLPesticides andRtx
®
-CLPesticides2Columns.
30m, 0.32mm ID, 0.50µmRtx
®
-CLPesticides and 30m, 0.32mm ID, 0.25µmRtx
®
-CLPesticides2 columns (cat.#’s 11139& 11324).
On-column concentration:
16-160pg;
Oven temp.:
80°C (hold 1min) to 300°C@ 10°C/min (hold 15min);
Inj. port:
Direct, Uniliner
®
sleeve (cat.# 20335);
Detector:
ECD, 300°CwithAnode Purge;
Dead time:
1.9min;
Head pressure:
8.7psi (constant);
Flow rate:
1.3mL/min@ 120°C, helium.
min. 10
15
20
1
2
25
In the environmental industry, the chlorinatedpesticide analytical
methods often are themost challenging toperform.Analysts
strugglewith linearity, breakdown, and lengthy calibrations; as
well as columnbleed, column reactivity, andpoor separation.
Restekhas addressedmanyof these issueswith the development
of theRtx
®
-CLPesticides and theRtx
®
-CLPesticides2 capillary
columns.These columnsweredesigned specifically for the
separationof chlorinatedpesticides, tobe used inparallel for
simultaneousquantitationandconfirmationbygas chromatogra-
phy/electron capturedetection (GC/ECD).Wehave reported the
performance and separation for these columns in several articles,
butmany laboratories are nowdealingwith the latest versionof
the chlorinatedpesticidesmethod—SW-846, 8081A.This
method adds several new target analytes to the20 common
single-component pesticides contained in earlier versions.
TheRtx
®
-CLPesticides andRtx
®
-CLPesticides2 achievebaseline
separationof the 20 chlorinatedpesticides listed inUSEnviron-
mental ProtectionAgency (EPA)Method8081 (Figure1). They
alsohave ahighmaximumoperating temperature, excellent
inertness, lowbleed, andoperate under the same flow and
temperature conditions. This facilitates installing them as a
columnpair into a single injectionport, therebyminimizing
maintenance concernswith the injectionports.
Because theRtx
®
-CLPesticides andRtx
®
-CLPesticides2 columns
weredesignedwith selectivity for neutral, halogenated com-
pounds, they are easily adapted for the analysis of the extended
list of pesticides inMethod8081A. Figure 1 also shows the
separationof thenine additional single-component compounds
listed inMethod8081A.Using a guard column and splitting the
1. dibromochloropropane
2. hexachlorocyclopentadiene
3. tetrachloro-
m
-xylene
4. hexachlorobenzene
5.
cis
-diallate
6.
α
-BHC
7.
trans
-diallate
8.
γ
-BHC
9.
β
-BHC
10.
δ
-BHC
11. heptachlor
12. aldrin
13. isodrin
14. heptachlor epoxide
15.
γ
-chlordane
16.
α
-chlordane
17. 4,4'-DDE
18. endosulfan I
19. dieldrin
20. chlorobenzilate
21. endrin
22. 4,4'-DDD
23. endosulfan II
24. 4,4'-DDT
25. endrin aldehyde
26. methoxychlor
27. endosulfan sulfate
28. endrinketone
29. decachlorobiphenyl
6,7
8
9
10
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16
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min. 10
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