RestekCorporation • (800)356-1688 • (814)353-1300 •
Figure 5 displays the analysis of aspirin and salicylic acid,
which also are easily separated on the Allure
™
Acidix phase
without the need of 1-heptane sulfonic acid or special solid
phase supports referenced by the USP. This separation uses
LC/MS compatible buffers and will out-perform the repro-
ducibility of any method employing ion-pair reagents.
Ibuprofen and naproxen are medications frequently recom-
mended for pain caused by inflammation. Figure 6 displays
the separation of these closely related carboxylic acids in
under seven minutes using an Allure
™
Acidix column.
The Restek Ultra Phenyl, Allure
™
Acidix, and Allure
™
Basix
columns provide excellent separation of analgesics without
the need of additional mobile phase modifiers. The Food and
Drug Administration (FDA) is now requiring “grandfathered”
methods that suffer from low accuracy and precision to be
upgraded to state-of-the-art methods. These phases provide
the means to update many of the older methods used in
pharmaceutical compendia, which will reduce laboratory
costs, improve laboratory productivity, and minimize
additive obstacles to LC/MS compatibility.
Figure4
Acetaminophen elutes with excellent peak shape in under 4 minutes using the Allure
™
Acidix column.
Figure5
Aspirin and salicylic acid are easily separated on the Allure
™
Acidix column.
1
Column:
Allure
™
Acidix
Catalog #:
9162565
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
60Å
Conditions:
Mobile Phase:
20mM ammonium
acetate pH 4.5:
acetonitrile
(95:5, v/v)
Flow:
1.0mL/min.
Temp.:
ambient
Det.:
UV @ 220nm
Peak List:
1. acetaminophen
Sample:
Inj.:
10µL
Conc.:
1mg/mL
Solvent:
water:acetonitrile
(1:1, v/v)
0 1 2 3 4 5 6 7 min.
1
2
0
2
4
6
8
10 min.
3
4
Column:
Allure
™
Acidix
Catalog #:
9162565
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
60Å
Conditions:
Mobile Phase:
20mM ammonium
acetate pH 4.5:
acetonitrile
(20:80, v/v)
Flow:
1.0mL/min.
Temp.:
ambient
Det.:
UV @ 220nm
Peak List:
1. toluene
2. unknown
3. salicylic acid
4. aspirin
Sample:
Inj.:
10µL
Conc.:
200µg/mL
Solvent:
buffer : acetonitrile
(1:1, v/v)
