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Figure 2 displays a four-component mixture of acetami-
nophen (a secondary amide); and codeine, oxycodone, and
hydrocodone (tertiary amines). Admixtures of acetami-
nophen and one of the above tertiary amines are used as
popular prescription painkillers. The Allure
™
Basix column
separates all these analytes within 8.5 minutes, showing
excellent resolution and peak shape without the need for the
docusate sodium modifier recommended in certain method
compendiums.
Acetaminophen also is included in many over-the-counter
remedies for congestion. In Figure 3, the popular mix of
nitrogen-based compounds, such as acetaminophen, pseu-
doephedrine, and caffeine are separated. The Allure
™
Basix
column performs well, separating all compounds in under 9.5
minutes with excellent shape and resolution, and without the
need of ethanesulfonic acid as an ion-pairing agent.
Some of the oldest analgesics are not bases, but acids.
Acetaminophen falls into the category of a weak acid. Figure
4 displays the analysis of acetaminophen on the Allure
™
Acidix column. The selective interaction of this column with
acidic solutes, such as acetaminophen, results in excellent
peak shape and an analysis time of under four minutes.
Figure2
The Allure
™
Basix column separates acetaminophen and tertiary amines within 8.5minutes,
resulting in excellent resolution and peak shape.
Figure3
The Allure
™
Basix column separates acetaminophen mixed with nitrogen-based compounds
in under 9.5 minutes, with excellent resolution and peak shape.
Column:
Allure
™
Basix
Catalog #:
9161565
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
60Å
Conditions:
Mobile Phase:
20mM ammonium
acetate, pH 4.5:
acetonitrile
(85:15, v/v)
Flow:
1.0mL/min.
Temp.:
30°C
Det.:
UV @ 280nm
Peak List:
1. acetaminophen
2. codeine HCl
3. oxycodone HCl
4. hydrocodone bitartrate
Sample:
Inj.:
5µL
Conc.:
100µg/mL
Solvent:
mobile phase
0 1 2 3 4 5 6 7 8 9 min.
1
2
3
4
1
Column:
Allure
™
Basix
Catalog #:
9161565
Dimensions:
150 x 4.6mm
Particle Size:
5µm
Pore Size:
60Å
Conditions:
Mobile Phase:
10mM ammonium
acetate, pH 6.9:
methanol
(75:25, v/v)
Flow:
1.0mL/min.
Temp.:
25°C
Det.:
UV @ 210nm
Peak List:
1. sample solvent (marker)
2. caffeine
3. acetaminophen
4. pseudoephedrine
Sample:
Inj.:
5µL
Conc.:
100µg/mL
Solvent:
mobile phase
2
3
4
0
2
4
6
8
10 12 min.
