SNAP
®
Series Glass Columns
Troubleshooting
Problem
Cause
Solution
1. Air Pockets
Solvent Evaporation or gas evolution
during storage
Recondition the column
2. Abnormal pressure fluctuations
during operation
1. Incorrect valve position
2. Blocked frit
3. Fitting tightened too much
1. Check valve position
2. Remove and dismantle piston,
replace frit, reassemble and re-
insert piston.
3. Recondition column. Replace fit-
tings and ferrules, re-cut the end
of the tubing
3. Column leaking solvents
See Diagram on Pg. 12
See Diagram on Pg. 12
4. Deteriorated Peak shape of elut-
ed substance
1. Bead bed mechanically damaged
2. Inlet frit partially blocked
3. Outlet frit partially blocked
4. Dead volume at column inlet
5. Contamination affecting separati-
on efficiency of stationary phase
1. Repack column
2. See 2.2 above
3. See 2.2 above
4. Rotate the SNAP
®
clamp counter-
clockwise until piston just contacts
bed
5. Repack column in sterile environ-
ment
5. Pressure drop during operation 1. Tubing or fitting leak between
pump and column
2. Solvent supply is dry
1. Check tubing/connections
2. Refill Solvent
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