SNAP
®
Series Glass Columns
Operating the Column with the Packing Adaptor
(continued)
Packing the Column:
In an external beaker using proper ventilation, mix the buffer and stationary phase until suspended in solution. Slowly add
the slurry to the inside wall of the glass column being careful not to trap air bubbles. Allow resin to sit until approximately a
centimeter of buffer sits on top of the bed. Position the packing adaptor and column so that they are perfectly vertical.
Wet the tip of the piston (Frit and O-Ring) in 20% ethanol to break the surface tension and allow for unrestricted flow. Insert the
piston just inside the resin volume. Make sure air bubble are not present. Position the nut so that it contacts the glass. Attach
the clamp and fasten SNAP
®
latch. Remove cap from outlet tubing and direct it to waste.
Turn on the pump to the appropriate flow rate and pressure. Slightly before the resin bed packs half of the desired length,
stop the pump. Disconnect inlet tubing and direct to waste. Cap the outlet tubing. Slowly turn the clamp counterclockwise. As
the piston submerges into the buffer, excess buffer will flow up the piston into the waste. Stop piston just above the resin bed.
If the bed has compressed to exist in only the column and not the packing adaptor, the packing adaptor can be removed by
opening the jaw and dismounted from the column. Uncap the outlet tubing directing it to waste. Insert the piston like stated
earlier and reconnect the inlet tubing to the controller unit
Once the bed compresses fully, stop the pump, cap the outlet tubing and unscrew the inlet tubing sending it to waste. Turn the
clamp counterclockwise until the frit just contacts the surface of the bed. Reconnect the inlet and outlet tubing to the control
unit. The column is ready to use.
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