SNAP
®
Series Glass Columns
Preparing the Column and Installation
Prior to first use of column, perform the following steps:
1.
Disassemble column completely by first unlatching the SNAP
®
s on top and bottom clamps
2.
Remove piston/nut assembly by pulling piston straight out
3.
Clean all components with soapy water or laboratory detergent. Always finish cleaning by thoroughly rinsing with
distilled water.
4.
Install supplied O-Ring on piston end
5.
Prior to reinserting pistons, frit must be “wetted out” with 20% ethanol to break surface tension and allow for
unrestricted flow
6.
When reinserting the piston(s), take care to insert straight into the glass body, otherwise the seal risks breakage
7.
Rotate the nut block until it contacts the glass
8.
For packing purposes, install the bottom clamp only, leaving top open for pouring media
Connecting the Column
Securely fasten the inlet and outlet connections to ensure that leakage does not occur during use.
Essential Life Solutions provides connections for either 1/16 or 1/8 inch tubing. The connection on the end of the piston is
standard ¼-28 FPLC flat bottom. Due to the fact that many systems standardize on M6, Essential Life Solutions has provided
adaptors to accommodate this condition.
Check your system’s standard threads to properly connect the column and avoid
cross threading.
Tubing should be connected to the SNAP
®
Columns with the following screw:
For tubing with 1/16” outer diameter:
ELS-JR-55050
Nut ¼”-28 G
ELS-JR-CFL-CB1KF-S
Ferrule, collapsible 1/16”
For tubing with 1/8” outer diameter:
ELS-JR-55051
Nut ¼”-28 G
ELS-JR-CFL-CB2KF
Ferrule, collapsible 1/8”
Two adapters with metric threads (M6) (ELS-P621) are also supplied with the
spare parts kit (ELS-1/16-KIT or ELS-1/8-KIT). These adapters are not suitable
for connection to the glass columns as they will damage the thread in the
pistons! They are to allow the columns to be connected to pumps, etc. fitted
with metric threads.
IMPORTANT!
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