1) N. Nagae, T. Enami and S. Doshi, LC/GC North America October 2002.
２
) T. Enami and N. Nagae, American Laboratory October 2004.
３
) T. Enami and N. Nagae, BUNSEKI KAGAKU, 53 (2004) 1309.
Sunniest C18
Sunniest RP-AQUA
Sunniest C8
Stability under basic pH conditions was evaluated at 50 ºC
using methanol/Sodium borate buffer pH 10 (30:70) as
mobile phase. Sodium borate is used as a alkaline standard
solution for pH meter, so that its buffer capacity is high.
Elevated temperature of 10 ºC makes column life be one
third. When Sunniest C18 column is used at 40 ºC, column
life becomes 2,000 hours. Other company shows stability
test at ambient (room temperature). If room temperature is
25 ºC, column life at room temperature (25 ºC) is sixteen
times longer than that at 50 ºC.
★
Sunniest C18 is enough stable even if it is used
under pH 10 condition. Regarding stability under
basic pH condition, there is little C18 column like
Sunniest C18 except for hybrid type C18. It is
considered that our end-capping technique leads
high stability.
★
Sunniest C18 can be used at the pH
range from 1.5 to 10. Sunniest C8 can be
used at the pH range from 1.5 to 9.
0
20
40
60
80
100
120
0
200
400
600
800
Sunniest C18, pH10 – 50ºC
Sunniest C8, pH9.2 – 40ºC
◆
Stability under acidic and basic pH conditions
Relative plate number of butylbenzene (%)
Time (h)
Durable test condition
Column: Sunniest C18, C8, 5
μ
m 150 x 4.6 mm
Mobile phase:
C18: CH
3
OH/20mM Sodium borate/10mM NaOH=30/21/49
(pH10)
C8: CH
3
OH/20mM Sodium borate (pH9.2) =30/70
Flow rate: 1.0 mL/min
Temperature: C18 - 50 ºC, C8 - 40 ºC
Measurement condition
Column size: 150 x 4.6 mm
Mobile phase: CH
3
CN/H
2
O=60/40
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Sample: 1 = Uracil
2 = Ethylbenzene
20
30
40
50
60
70
80
90
100
0
20
40
60
80
100
120
Time (h)
Relative retention of ethylbenzene (%)
Sunniest C18
Sunniest RP-AQUA
Sunniest C8
B company C18
D company C18
F company C18
Durable test condition
Column size: 150 x 4.6 mm
Mobile phase: CH
3
CN/1.0% TFA (pH1) = 10/90
Flow rate: 1.0 mL/min
Temperature: 80 ºC
Measurement condition
Column: Sunniest C18, C8, 5
μ
m 150 x 4.6 mm
Mobile phase: CH
3
CN/H
2
O=75/25
Flow rate: 1.0 mL/min
Temperature: 40 ºC
Sample: 1 = Butylbenzene
Stability under acidic pH conditions was evaluated at 80
ºC using acetonitrile/1% trifluoroacetic acid solution
(10:90) as mobile phase. 100% aqueous mobile phase
expels from the pore of packing materials by capillarity
and packing materials doesn’t deteriorate. 10%
acetonitrile in a mobile phase allows an accurate
evaluation.
1-3)
★
Sunniest C18 has kept 90% retention for 100
hours under such severe conditions.
Our bonding technique can make column life be
long.
Sunniest RP-AQUA is less stable than Sunniest
C18. However, Sunniest RP-AQUA has more
stable than other company C18 columns.
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