Sunrise C18 and C18-SAC
Silanol Activity Controlled C18 HPLC Column
Sunrise C18
Sunrise C18-SAC
•The “1st Choice” column as a fully end-capped C18 column
•Full end-capping after silanol activity control
•Reducing
ng
adsorption of
adsorption o
a basic compound extremely
•A good peak shape for a metal cheleting compound
•Widely available for general reversed-phase separation
•The "2nd Choice" column which takes advantage of
effective silanol groups interaction
•Reducing silanol groups with high adsorption activity
•The new separation mechanism including hydrogen bond
and ion-exchange interaction
•Effective for separation of a basic compound and a polar
compound
•Different selectivity and improvement of separation without
changing a mobile phase
■
The elution order of pyridine
It is generally said that residual silanol groups on
a stationary phase such as C18 (ODS) causes
absorption or peak tailing for a sample.
Especially silanol groups near a hydrophobic
site don
’
t solvate with water completely, so that
they show high absorption for basic compounds.
Its peak shows terribly tailing. Several end-
capping techniques have been developed to
solve these problems for many years.
◆
New generation reversed-phase utilized silanol groups
◆
Feature of Sunrise series
1st generation
4th generation
3rd generation
2nd generation
Pellicular type
Bonded type
High carbon and
end-capped type
Hybrid type
(Post-X2)
Sunrise
C18-SAC
Highly end-
capped type
5th generation
Silanol Activity
Control
Technology
Sunrise C18
ChromaNik developed the technique that
decreased only silanol groups with high
absorption activity to a basic compound and
remained effective sailnol groups on the
stationary phase. Silanol activity control and no
end-capping led the existence of silanol groups
with high hydration which created a new and
unique reversed-phase separation mode
including hydrogen bond and ion-exchange
interaction. Furthermore, silanol activity
controlling, then end-capping technique
improved a peak shape of a basic compound
exceedingly.
■
Silanol group and peak tailing
■
Silanol activity control technology
Silanol activity control
O
Si
H
O
Si
H
O
Si
O
O
Si
Si
H
O
Si
H
O
Si
O
O
Si
Si
Si
Silanol groups
with high
absorption activity
Changeover to
siloxane bond
Silanol Activity
Control
Technology
C) Sunrise C18-SAC
(non end-capped)
1
1
3
3
0
5
10
15
20
retention time / min
(2)
(2)
A) Sunrise C18
(end-capped)
Column size: 4.6 X 150mm
Mobile phase:
30/70= CH3OH/H2O
Sample: 1 = uracil
(2) = pyridine
3 = phenol
B) Conventional no end-capped C18
1
(2)
3
